Sil Lab – Junior/Assistant

Key Responsibilities

• Conducting molecular and cell biology experiments, such as PCR, qPCR, in vitro transcription, cloning, gel electrophoresis, immunoprecipitations, Northern and Western blotting
• Aseptically culturing fungal cell lines and performing genetic manipulations using CRISPR-Cas9
• Preparing RNA and DNA libraries for next-generation sequencing
• Microscopic analysis of fungal cells
• Collecting, analyzing, and presenting experimental data

Required Qualifications

• Excellent organizational and interpersonal communication skills (verbal and written)
• Willingness and ability to learn new methods and skills for changing research priorities
• Ability to work independently and as a member of a research team
• Ability to prioritize tasks, coordinate work tasks with others, and meet multiple deadlines
• Must have (or be in process of obtaining) a bachelor’s degree (or equivalent degree) or four years of research experience to be appointed at the Junior rank
• Must have (or be in process of obtaining) a master’s degree (or equivalent degree) or a bachelor’s degree with three or more years of research experience to be appointed at the Assistant rank

Preferred Qualifications

• Prior experience with handling pathogenic microbes, aseptic techniques
• Prior experience with BSL2/BSL3 biocontainment
• Prior experience in the design and assembly of DNA constructs
• Prior experience in molecular genetics and biology (i.e. gene knockouts, epitope tagging, immunoprecipitation, protein-protein interactions)
• Prior experience with RNAseq and library preparation
• Familiarity with RNA biology, gene expression assays, Western blotting
• Prior experience working in a research lab with fungi