Scientist -Antibody Technologies - Exp in Protein expression, Tissue culture, Molecular biology

Position : Scientist -Antibody Technologies - Required Exp in Protein expression, Tissue culture, Molecular biology techniques Location : San Diego, CA Duration : 12 Months Total Hours/week : 40.00 pay rate:$20-30/hr 1st Shift Client : Medical Devices Company Job Category : Research & Development Level of Experience : Entry - Level Employment Type : Contract on W2 (Need US Citizens or GC Holders or GC EAD or OPT or EAD or CPT) Work hours: 40 hrs/wk. standard work hours ~8am-5pm. 3 Must haves on the resume: min 3 years lab experience. Protein expression experience. Tissue culture experience. Molecular biology techniques. Job Description We are seeking a highly motivated and organized Scientist III to contribute to the discovery and development of monoclonal antibodies in our Antibody Discovery team. This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams, including but not limited to protein expression and purification, cell-line engineering and testing new antibodies for use in flow cytometry application. The position requires extensive hands-on experience with protein expression, tissue culture, and a strong understanding of biochemistry and molecular biology techniques. The successful candidate is expected to set up and execute experiments, be detail-oriented, skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results. Duties And Responsibilities Responsible for transfection and expression of antibodies and recombinant proteins using multiple cell lines and expression systems.Responsible for sterile cell culture of multiple cell lines using aseptic technique for expression and flow testing.Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques.Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.Involvement with scheduling, operational and maintenance duties required within the laboratory. Knowledge And Skills Protein expression using various systems including but not limit to mammalian cells, insect cells, and bacteria.Proficient with aseptic technique and cell-culture techniques.Protein purification with liquid chromatography and quality assurance using various analytical methods.Preferred: Familiarity with assay automation and automated liquid handlers.Preferred: Experience with cell line engineering.Preferred: Hand-on experience with Flow Cytometry and the ability of analyzing flow data.Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.Effective written and oral communication skills.Demonstrated ability to follow established policies and procedures.Demonstrated ability to keep neat, accurate, and complete records.Effective analytical and problem-solving skills. Education And Experience A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 3-6 years of laboratory experience required.Protein expression, molecular biology and/or tissue culture experience required.