Scientist -Antibody Technologies - Required Flow Cytometry, Tissue Culture & lab Exp)

Duties and Responsibilities

• Responsible for purification of antibodies and recombinant proteins using AKTA FPLC and manual methods.
• Executes analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR.
• Responsible for transfection and/or expression of antibodies and recombinant proteins from various mammalian cell lines, including but not limited to CHO and hybridomas as well as E. coli.
• Responsible for the execution of experiments with varying complexity using advanced molecular biology techniques.
• Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge and Skills

• Protein purification with liquid chromatography and quality assurance using various analytical methods.
• Protein expression using various systems including but not limited to mammalian cells, insect cells, and bacteria.
• Proficient with aseptic technique and cell-culture techniques is required.
• PREFERRED: Familiarity with assay automation and automated liquid handlers.
• PREFERRED: Experience with cell line engineering
• PREFERRED: Hand-on experience with Flow Cytometry and the ability of analyzing Flow data is preferred.
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Demonstrated ability to think analytically and solve problems.

Education and Experience

• A Bachelor’s or Master of Science degree in biochemistry, cellular or molecular biology, immunology, or related discipline and a minimum of 2-4 years of laboratory experience required.
• Protein purification, protein expression, molecular biology and tissue culture experience required.