Biology Scientist II-Antibody Technologies (Req Exp in Flow Cytometry, FCS & Tissue Culture) -Onsite

Position

Biology Scientist II - Antibody Technologies

About the role

We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Technologies team.

Responsibilities

• Purification of antibodies and recombinant proteins using AKTA FPLC and manual methods
• Execute and document work using SOPs and follow strict GMP requirements under ISO 13485 standards
• Coordinate preparation and transfer of purified antibody and protein samples to other functional teams
• Execute analytical testing of purified antibodies and proteins using SEC, SDS-PAGE, Western blot, MADLS, and qPCR
• Transfect and/or express antibodies and recombinant proteins from various mammalian cell lines, including CHO and hybridomas as well as E. coli
• Execute experiments with varying complexity using advanced molecular biology techniques
• Execute experiments with varying complexity using multi-color Flow Cytometry
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments
• Efficiently prioritize individual workload
• Involvement with scheduling, operational and maintenance duties required within the laboratory

Requirements

• Extensive hands-on experience with protein purification, tissue culture, protein expression, and a strong understanding of biochemistry and molecular biology
• Detail-oriented, skilled at data analysis and troubleshooting
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals
• Effective written and oral communication skills
• Demonstrated ability to follow established policies and procedures
• Demonstrated ability to keep neat, accurate, and complete records
• Demonstrated ability to follow established policies and procedures
• Demonstrated ability to follow established policies and procedures

Skills

• Protein purification with liquid chromatography and quality assurance using various analytical methods
• Protein expression using various systems including but not limited to mammalian cells, insect cells, and bacteria
• Proficient with aseptic technique and cell-culture techniques
• Familiarity with assay automation and automated liquid handlers (preferred)
• Experience with cell line engineering (preferred)
• Hand-on experience with Flow Cytometry and the ability of analyzing Flow data (preferred)

Education and Experience

• A Bachelor’s or Master of Science degree in biochemistry, cellular or molecular biology, immunology, or related discipline
• A minimum of 2-4 years of laboratory experience required
• Protein purification, protein expression, molecular biology and tissue culture experience required