Scientist II - Req Exp in Flow Cytometry, tissue culture

Duties And Responsibilities

• Design and execute experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Transfect and express antibodies in cell lines, conduct hybridoma tissue culture, and culture various cell lines for testing.
• Execute experiments using automated liquid handling and other automation equipment.
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Contribute to identification and implementation of process improvement projects.
• Train junior scientists on laboratory techniques and business processes.
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments.
• Efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge And Skills

• Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
• Familiarity with assay automation.
• Familiarity with molecular biology (DNA preparation).
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Demonstrated ability to think analytically and solve problems.

Education And Experience

• A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-4 years of laboratory experience required.
• Flow cytometry and tissue culture experience required.