Research Associate II/III - R&D

Responsibilities

• Perform molecular biology experiments including cloning, PCR, and nucleic acid purification
• Isolate plasmid DNA (Miniprep to Maxiprep scale) and verify the sequences
• Generate phage display libraries and perform panning experiments with multiple targets
• Express and purify recombinant proteins from eukaryotic and/or prokaryotic hosts
• Characterize purified proteins using a variety of biochemical techniques (UV/Vis, SDS-PAGE, ELISA, surface plasmon resonance (SPR), etc.)
• Prepare buffers, reagents, and other materials necessary for experiments
• Accurately record experimental data in a timely manner
• Properly label and store all samples, buffers or reagents
• Other duties as assigned

  Requirements

  • Basic Qualifications
  • B.S. in a biology related discipline with 2+ years of experience in an academic or industry setting
  • Experience with molecular cloning, DNA primer design, and PCR methodologies
  • Experience with DNA/RNA purification (e.g., Miniprep or Maxiprep) and gel electrophoresis
  • Experience working with phage and/or performing phage display experiments (library construction, panning, and sequencing)
  • Hands-on experience developing and optimizing ELISA assays
  • Ability to work independently, interpret data, and follow written procedures/protocols precisely
  • Good communication, interpersonal, and teamwork skills
  • Highly organized and committed to integrity, professionalism, and collegiality
  • Preferred Qualifications
  • Prior experience with protein/antibody characterization (SDS-PAGE, bio-layer interferometry (BLI), and surface plasmon resonance (SPR))
  • Familiarity with AKTA FPLC instruments and Unicorn software suite for protein purification
  • Familiarity with toxins and/or infectious pathogens
  • Familiarity with aseptic technique and mammalian cell culture
  • Familiarity with Next-Generation Sequencing (NGS) sample preparation and downstream data analysis