Specialist, Cell Line Engineering (READY Center) – Junior, Assistant, Associate, Full Specialist

Key Responsibilities

• Contribute to the design and implementation of advanced genome engineering strategies using CRISPR/Cas systems, including knockouts, targeted knock-ins, landing pad systems, and gene expression modulation approaches.
• Perform and support genome editing workflows in human cell systems, including iPSCs, with attention to experimental quality, efficiency, reproducibility, and scalability.
• Execute molecular biology techniques such as cloning, nucleic acid extraction, PCR, and sequencing-based validation to support cell engineering projects.
• Support the establishment and maintenance of workflow for clonal isolation, genotyping, and validation of engineered cell lines.
• Aid in process development efforts to standardize and scale genome engineering workflows.
• Integrate automation and high-throughput technologies (e.g., liquid handling systems) into cell engineering and assay workflows to increase throughput and data quality.
• Contribute to scaling and standardization of workflows, including participation in process improvement and troubleshooting efforts.
• Collaborate closely with multidisciplinary teams to build platform capabilities.
• Maintain accurate and well-organized experimental records and contribute to data analysis and reporting.

Required Qualifications

• Junior Specialist - Baccalaureate degree (or equivalent degree) in Molecular Biology, Genetics, Bioengineering, or related field or at least four years of research experience
• Assistant Specialist - Master’s degree or a baccalaureate degree in Molecular Biology, Genetics, Bioengineering, or related field with 3 or more years of research experience
• Associate Specialist - Master’s degree (or equivalent degree) in Molecular Biology, Genetics, Bioengineering, or related field or five to ten years of experience in the relevant specialization
• Full Specialist - terminal degree (or equivalent degree) in Molecular Biology, Genetics, Bioengineering, or related field or ten or more years of experience in the relevant specialization
• Hands-on experience with mammalian cell culture.
• Practical knowledge of molecular biology techniques (e.g., PCR, nucleic acid extraction, cloning).
• Exposure to genome engineering approaches such as CRISPR/Cas systems or related technologies.
• Able to maintain clear and organized experimental documentation.
• Strong problem-solving skills and attention to detail.
• Able to work both independently and collaboratively in a team-oriented research environment.
• Effective communication skills and willingness to learn new techniques.

Preferred Qualifications

• PhD or Master’s degree in Molecular Biology, Genetics, Bioengineering, or related field, or with 5+ years of relevant laboratory experience; or equivalent combination of education and experience.
• Hands-on experience with CRISPR/Cas genome editing in mammalian systems, including knockout, knock-in, or gene regulation approaches.
• Experience working with human induced pluripotent stem cells (iPSCs), including maintenance, editing, and differentiation, particularly neural or disease relevant lineages.
• Experience developing high-throughput or automated genome engineering pipelines using liquid handling systems (e.g., Hamilton).
• Experience with NGS-based methods for assessing genome editing outcomes, including off-target analysis (e.g., GUIDE-seq, CIRCLE-seq, amplicon sequencing).
• Experience developing or optimizing workflows for reproducibility, scalability, or throughput (e.g., process development, screening platforms).
• Programming or data analysis experience (e.g., Python, R) for analysis of sequencing or screening datasets.
• Prior experience mentoring or leading junior team members.