Senior Principal Scientist - Bioanalytical Research

Primary Responsibilities

• Perform sample preparation (e.g. tissue homogenization, multiwave digestion, immunoaffinity enrichment, protein precipitation) and operate mass spectrometer to develop bioanalytical methods and perform quantitative bioanalysis (bioA) of small molecule or/and large molecule therapeutics from in-vitro and in-vivo samples.
• Investigate the structure-activity relationship (SAR) including pharmacokinetics (PK), biodistribution (bioD), biotransformation (BioT) and metabolite identification (metID) of drug molecules using LC-MS/MS.
• Collaborate with ADME project leaders to support fast-paced drug discovery projects. Serve as bioA representative and provide bioanalytical expertise to project team.
• Mentor junior scientists in developing their skills in LC-MS sample preparation, data analysis, visualization and reporting.
• Maintain and troubleshoot lab instruments to ensure their operation.

Job Requirements

• Bachelor’s degree in science or engineering (chemistry, chemical engineering, bioengineering or related field) with at least 7 years of work experience, or master’s degree in related field with at least 5 years of work experience.
• Experience with using inductively coupled plasma (ICP), triple quadrupole (QQQ), high-resolution mass spectrometer (HRMS, including QToF, Orbitrap) in bioA method development, qualification, and transfer.
• Experience with small and large molecule bioanalysis, including sample preparation (e.g. tissue homogenization, microwave assisted acid digestion), sample extraction (LLE, SPE, immunoprecipitation, protein precipitation) prior to MS.
• Experience of drug ADME principles in understanding PK, biodistribution and biotransformation of small and large molecule therapeutics.
• Qualified applicants must be authorized to work in the United States on a full-time basis. Lilly will not provide support for or sponsor work authorization or visas for this role, including but not limited to F-1 CPT, F-1 OPT, F-1 STEM OPT, J-1, H-1B, TN, O-1, E-3, H-1B1, or L-1.

Preferred Qualifications

• Experience with discovery and development of radioligand therapeutics and elemental analysis using Agilent 8900 ICP-MS. Hands-on experience in sample preparation, LC-MS bioanalytical workflow to support the bioA and metID of RLTs.
• Familiar with bioanalytical workflow of biotherapeutics (mAbs, ADC, siRNA, fusion protein, etc.), including immunoprecipitation, proteolytic digestion and deglycosylation. Bottom-up peptide, payload quantification using Sciex 6500/7500. Intact mass acquisition, top-down MS using Thermofisher Orbitrap Exploris 240/480/Tribrid instruments.
• Familiar with MS-related informatic tools to conduct in-depth data analysis – Mass Hunter, Biopharma Finder, QuantBrowser, MultiQuant, Biologics Explorer, Protein Metrics, MassMetaSite, etc. AI-based data analysis and report writing are an advantage.
• Previous experience in experiment documentation (eLN, signals, benchling, etc.), PK calculation (Phoenix, Watson LIMS, R-studio aNCA), data visualization (PRISM, BioRender) and report QC.
• Hands-on experience in automation instruments, such as Apricot, Hamilton, Tecan and KingFisher to support high-throughput bioanalytical assays.

Pay

Actual compensation will depend on a candidate’s education, experience, skills, and geographic location. The anticipated wage for this position is $71,250 - $213,400. Full-time equivalent employees also will be eligible for a company bonus (depending, in part, on company and individual performance).

Benefits

Lilly offers a comprehensive benefit program to eligible employees, including eligibility to participate in a company-sponsored 401(k); pension; vacation benefits; eligibility for medical, dental, vision and prescription drug benefits; flexible benefits (e.g., healthcare and/or dependent day care flexible spending accounts); life insurance and death benefits; certain time off and leave of absence benefits; and well-being benefits (e.g., employee assistance program, fitness benefits, and employee clubs and activities).