Scientist - Yen Lab/TGEn Group (Hematopoietic Stem Cell, Genome Editing)

Job Responsibilities

• Perform studies to develop genome editing strategies for human hematopoietic stem cells (HSC).
• Design and execute IND-enabling experiments to characterize the pharmacology of a genome-edited human hematopoietic stem cell product to treat sickle cell disease.
• Characterize the efficacy, safety, and potency of the HSC cellular drug product in in vitro and xenotransplantation assays.
• Multicolor flow cytometry and FACS to sort hematopoietic populations of relevance.
• Develop experimental methods including RT-PCR, ddPCR, next-generation sequencing, and single-cell sequencing.
• Perform NGS library preparation and analysis of editing outcomes, on-target and off-target.
• Evaluate scale-up and close-system processes to ensure reproducibility of the drug product.
• Conduct exploratory research on developing novel in-vitro and in-vivo delivery strategies.
• Interact and collaborate with multidisciplinary teams to advance the program.
• Establish tracking and trending analyses of process development and pilot runs.
• Draft documentation (protocols, batch records, reports) to standardize and transfer process to GMP facility.
• Maintain regular and predictable attendance.
• Perform other related duties as assigned to meet the goals of the department and institution.

Minimum Education and/or Training

• Bachelor’s degree in a relevant scientific area is required.
• Master’s or PhD degree in a relevant scientific area is highly preferred.

Scientist Minimum Experience

• Eight (8) years of relevant post-degree work experience is required with a Bachelor's degree.
• Six (6) years of relevant post-degree work experience is required with a Master's degree.
• Three (3) years of relevant post-degree work experience is required with a PhD.

Experience

• Experience leading and supporting research experiments preferred.
• Experience in guiding and training other laboratory personnel preferred.
• Proven performance in earlier role/comparable role.

Location

This position is based in Memphis, TN; relocation assistance is available.

Company Information

Welcome to Memphis. Memphis is a family-friendly metropolitan area that more than a million residents call home. Memphis is recognized for its world-class music, food, and hospitality. Cost of living is one of our biggest attractions; in fact, Money listed Memphis as one of the least expensive cities in which to raise a child. Mild winters and beautiful green spaces make the city a magnet for outdoor enthusiasts – there are over 200 miles of bike-friendly lanes with more being added. Forbes recently named Memphis the 4th happiest city in which to work.

Compensation

In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $86,320 - $154,960 per year for the role of Scientist - Yen Lab/TGEn Group (Hematopoietic Stem Cell, Genome Editing).