Scientist- (Cell Therapy & Gene Editing)

About the role

Advance the future of cell therapy and gene editing in a leading biotech organization in South San Francisco, CA. Play a key role in engineering next-generation cell therapies using CRISPR, lentiviral, and PiggyBac systems, iPSCs, and primary cells.

Responsibilities

• Support the needs of the ACE Hub, particularly in the engineering and profiling of cells for cell therapy development and disease modeling used across clients.
• Generate engineered cells (including cancer cell lines, iPSCs, and primary cells) using stable integration (both lentivirus and piggybac) and CRISPR gene editing.
• Transfection/gene editing optimization
• Troubleshoot cell engineering protocols
• Characterize engineered cells using genomics and molecular assays
• Maintain and quality check cell lines in culture

Requirements

• A self-motivated candidate with a BS/MS + 5 years industry experience, or a PhD in Molecular Biology, Cell Biology, Genetics, or a related discipline.
• Demonstrable strong Mammalian Cell Culture techniques and the ability to generate high-quality data.
• Excellent written and verbal communication skills and will be required to analyze and present data at team meetings.
• Team-oriented, highly organized both operationally and in silico, with the ability to multitask across a diverse array of projects, and eager to learn new skills.

Qualifications

• Authorized to work in the United States.
• Prior experience with primary T cells and iPSCs.
• Prior expertise in some or all of the following: DNA/RNA/Protein extraction and quantification, molecular cloning, PCR, RT-PCR, ddPCR assay design and screening, FACS, transfection, CRISPR genome editing, siRNA/shRNA, SDS-PAGE, immunoblotting, cell-based assays, NGS, and viral vector development/use.
• Experience with sequence/data analysis platforms such as Benchling, Snapgene, FlowJo, GraphPad Prism etc. are desirable.

Benefits

Pride Health and its affiliates offer eligible employees comprehensive healthcare coverage (medical, dental, and vision plans), supplemental coverage (accident insurance, critical illness insurance and hospital indemnity), 401(k)-retirement savings, life & disability insurance, an employee assistance program, legal support, auto, home insurance, pet insurance and employee discounts with preferred vendors.

Pay

$55-63/HR, depending on experience

Schedule

12-month contract opportunity with possibility to extend, fully onsite (5 days/week), Monday-Friday (40 hours/week)