Scientist, In-Vivo Large Construct Integration

Key Responsibilities

• Innovate and iterate on Neion Bio’s transgenic animal platform
• Develop and improve methods for DNA assembly, integration, editing, screening
• Develop and validate novel vertebrate genetic engineering approaches
• Assist in building and optimizing molecular and cellular tools for DNA/RNA delivery and perform high-throughput screens of these tools, including LNPs, VLPs, AAVs, lenti- and adenoviral vectors, electroporation and/or chemical transfection in cell culture, organ explants, live embryos and animals
• Characterize and quantify genome editing events using various molecular assays

Required Qualifications

• PhD/MS in synthetic biology, bioengineering, molecular biology, genetics or related field is required, or 3+ years hands-on expertise in genomic manipulation and cell biology
• Understanding and extensive hands-on experience with current precision editing techniques, covering the design, optimization and verification of edits made with CRISPR-Cas9/HDR and Prime Editing
• Experience with recombinases and integrases, or other large construct integration methods
• Experience with in-vivo delivery of large payloads for genomic integration
• Experience with avian or mammalian cell culture, DNA/RNA transfection, bacterial culture, recombinant DNA cloning techniques, primer design and PCR, qPCR, gel electrophoresis, nucleic acid extraction and purification
• Self-motivated, proactive, works well under pressure and deadlines, detail-oriented
• Enthusiasm for learning new skills and taking on new responsibilities
• Strong written and oral communication skills, as well as creative problem-solving skills
• Strong emotional intelligence and passion for building relationships across all levels of an organization.
• Enjoys the fast-paced startup environment and teamwork

Preferred Qualifications

• High throughput screening experience or automation methods
• Experience with viral vector design, optimization and use
• Experience with -omics based analysis of cells culture
• Experience with development of gene therapy tools
• Experience with flow cytometry, immunostaining, and fluorescent microscopy
• Experience with dPCR and/or NGS library preparation

Compensation

• Base salary is expected to be $110-150K depending on experience, with additional bonus compensation, equity compensation, and benefits including health insurance, dental insurance, vision insurance, and 401(k).