Scientist II, Protein Expression and Purification

Location: San Diego, CA | Job Type: Full-Time

About the role

We are seeking a Scientist II, Enzyme Assay & Characterization to play a key role in developing and executing biochemical assays that support the company’s multi-omics platform development. This individual will optimize and implement assays, contribute directly to enzyme evaluation, and engage in broad technical growth opportunities including assay development, molecular biology, protein engineering, and integration. The ideal candidate is detail-oriented, analytical, collaborative, and thrives in a dynamic environment that values innovation, cross-disciplinary interaction, and scientific rigor.

Responsibilities

• Clone, express, and screen protein constructs in bacterial expression systems.
• Optimize bacterial expression conditions.
• Execute purification workflows.
• Characterize protein quality.
• Optimize and implement biochemical assays.
• Develop, optimize, and validate spectroscopic and fluorescence-based assays.
• Troubleshoot and refine assays for reproducibility, sensitivity, and throughput.
• Collaborate closely with protein expression/purification, enzymology, protein engineering, and molecular biology teams.
• Document methods, maintain rigorous records, contribute to SOPs, and prepare data summaries and reports.
• Support lab organization, maintain equipment, and ensure compliance with safety and reproducibility practices.

Qualifications

• B.S. in Biochemistry, Molecular Biology, Enzymology, or related field with 4–6 years of relevant experience, OR M.S. with 2–4 years of relevant experience.
• Demonstrated experience optimizing and implementing biochemical assays.
• Hands-on experience with spectroscopic and fluorescence-based assays.
• Hands-on experience with gel electrophoresis and gel-based assays.
• Strong experience with data visualization software.
• Excellent organizational, problem-solving, and communication skills.
• Ability to work independently and collaboratively in cross-functional teams.

Benefits

• Competitive compensation and equity package.
• Comprehensive benefits.
• Flexibility to support work-life integration.

Pay

$110,000 to $120,000

Why Join Us

• Influence the foundational technologies and strategies of a company poised to shape the future of clinical genomics and healthcare.
• Work in a dynamic, collaborative environment where innovation and scientific rigor are deeply valued.
• Join a seasoned and multidisciplinary team tackling high-impact problems at the intersection of science and engineering.