Scientist I, Protein Purification & Expression

About the role

Nurix Therapeutics, Inc. is seeking a detail-oriented and experienced Protein Expression Scientist to join the DEL and Protein Sciences team. This individual will work cross-functionally with internal stakeholders to plan, coordinate, and execute all operational activities required for analytical scale and preparative scale expression of recombinant proteins in baculovirus and mammalian cell systems. Additionally, they will be responsible for documentation of expression conditions and results in internal databases and electronic lab notebook systems.

Responsibilities

• Highly proficient in maintaining insect cell and mammalian cell cultures.
• Excellent knowledge and high degree of familiarity with all steps in baculovirus expression of recombinant proteins, including clone design, generation and purification of bacmids, raising and titering of baculovirus, conducting MOI studies, cell culture of Sf9 and/or Tni/Hi5 cells, and harvesting of biomass on the scale of 1 L – 20 L.
• Strong working knowledge of protein characterization steps, including SDS-PAGE analysis, size exclusion chromatography, and/or thermal stability (e.g., nanoDSF).
• Past experience purifying recombinant proteins (e.g., affinity chromatography, ion exchange, or size exclusion chromatography) is a bonus.

Requirements

• Ph.D. or equivalent with 2+ years of relevant experience.
• 2+ years of experience working with insect cell lines and baculovirus expression of recombinant proteins.
• Additional experience working with recombinant protein expression in mammalian cells (e.g., HEK293) is a plus.
• Industry experience preferred with exemplified ability to overexpress milligram amounts of protein for structural and functional studies for multiple protein classes.
• Able to work in a dynamic, fast-paced environment with experience prioritizing work across multiple projects.
• Detail-oriented with strong record-keeping skills.
• Must be able to lift 50+ pounds comfortably.
• Onsite position.

Pay

Salary Range: $125,000 - $151,000 plus bonus and equity.

Fit with Nurix Culture and Values

• Strong team orientation; highly collaborative.
• Solutions and results-oriented focus.
• Hands-on approach; resourceful and open to diverse points of view.