Scientist II - Antibody Technologies

Duties And Responsibilities

• Responsible for transfection and expression of antibodies in cell lines, hybridoma tissue culture, and culture of various cell lines for testing. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques.
• Responsible for the execution of experiments with varying complexity using multi-color Flow Cytometry.
• Analyze data using FCS Express and FlowJo software, report test results including completion of all required documentation and database entries as required by protocols and SOPs.
• Assist with protein conjugation and molecular biology processes.
• Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met.
• Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload.
• Involvement with scheduling, operational and maintenance duties required within the laboratory.

Knowledge And Skills

• Proficient in tissue culture techniques; including transfection of cells for protein expression.
• Hands on experience designing, performing, and analyzing flow cytometry experiments, preferably on BD flow cytometers (LSRII, LSR Fortessa, Canto, Aria).
• Software: BD FACS Diva, FlowJo, FCS Express, and Microsoft Office Suite.
• Familiarity with molecular biology (DNA preparation)
• PREFERRED: Familiarity with assay automation.
• Effective interpersonal skills and ability to work in a cross-functional team-oriented environment to achieve organizational goals.
• Effective written and oral communication skills.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, and complete records.
• Effective analytical and problem-solving skills.

Education And Experience

• A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology, cell biology or related scientific discipline and a minimum of 2-5 years of laboratory experience required.
• Flow cytometry and/or tissue culture experience required.