Research Associate/Senior Research Associate, In Vivo Pharmacology

About the role

Thymmune Therapeutics is a preclinical biotechnology company based in Cambridge, MA applying proprietary insights in thymic engineering to develop and commercialize immune cell therapy products addressing significant unmet clinical need across immunology. Building on work from leading labs, Thymmune has strong IP positioning and is backed by early investors and founders of successful high-profile biotechnology companies.

Key Responsibilities

• In vivo pharmacology study
• Execute in vivo studies using selected mouse models to assess the efficacy and tumorigenicity risk of thymic cell product following engraftment.
• Perform standard dosing procedures (SC, IM, IV, IP) for cell administration, blood sampling techniques (submandibular bleeds and tail nick) for immune cell profiling, as well as tissue collection (e.g., lymph tissue, muscle, and major organs) via necropsy.
• Conduct and/or assist with small rodent surgical procedures (e.g., tissue transplantation, cells implantation), experience in surgical procedures is a key asset.
• Perform specialized tissue processing and in vivo imaging analysis, including immunohistochemistry (IHC) and IVIS-based reporter gene imaging.
• Collaborate cross-functionally to support timely execution of in vivo studies to characterize and select candidate cell therapy product.
• In Vitro biological & Immunological assays
• Perform cell isolation from primary human or mouse tissues to support downstream analysis.
• Conduct downstream biological assays to evaluate cell quality and function, including Flow Cytometry (FACS), RT-qPCR/ ddPCR, MSD/ELISA, Immunofluorescence imaging.
• Identify and validate target markers for cell profiling and apply these markers to correlate in vitro characteristics with in vivo outcomes.
• Maintain cell culture of cell line or primary immune cells using aseptic techniques.
• Develop and execute immunological assays (e.g., cell activation, cytotoxicity, mixed lymphocyte reaction) to assess the allo-reactivity and tolerance of immune cells.
• Data Analysis, Reporting, & Collaboration
• Document in vitro and in vivo studies using electronic notebook (e.g., Benchling) to maintain detailed records of study design, experimental procedure, analytical data, and results.
• Analyse complex datasets using statistical software (e.g., GraphPad Prism) and effectively present findings to the scientific team.
• Maintain high standards of data integrity and ensure accurate, reproducible, and well-documented data to support reliable analysis and decision-making.
• Contribute to the writing of study reports and participate in the strategic planning of long-term studies.
• Collaborate with cross-functional teams, provide training to junior laboratory staff and interns, fostering a collaborative and productive working environment.

Required Qualifications and Skills

• Education: B.S. or M.S. in Cell Biology, Immunology, Regenerative Medicine, or a related discipline.
• Experience: 1–3+ years of relevant experience (Biotech/Pharma experience preferred).
• In Vivo Skills: Proficiency in rodent handling, multiple routes of administration (IV, IP, SC), and tissue dissection is required.
• In Vitro Skills: Hands-on experience with the isolation and culture of primary human cells or tissues is preferred.
• Analytical Skills: Strong experience with Flow Cytometry, qPCR, and microscopy. Ability to analyse data statistically and troubleshoot experimental variances independently.
• Other Skills: Excellent oral and written communication skills. Must be able to structure tasks, set priorities, and work effectively in a collaborative, team-oriented environment.