Research Associate / Senior Research Associate, In Vitro Pharmacology

Responsibilities

• Run established in vitro shuttle screening assays in a variety of cell types; responsibilities will include cell culture, treatments, harvesting and downstream molecular biology readouts
• Maintain a variety of cell lines, including immortalized cell lines, primary cells and iPSC-derived cells
• Develop and execute program-specific assays for characterization/ testing of lead candidates and understanding MoA of shuttles and drug conjugates
• Support the characterization of lead shuttles by conducting receptor internalization/ trafficking assays, on-cell binding assays and/or ligand competition assays
• Support oligonucleotide programs through testing and qualification of reagents in disease program-specific cell types (e.g. qPCR primer/probe validation, antibody validation for western blots and ELISAs, siRNA sequence validation)
• Follow protocols rigorously and contribute to protocol refinement when needed
• Maintain accurate and organized data entry and analysis
• Collaborate with cross-functional teams in a dynamic research environment

Required Qualifications

• Bachelor's or Master's degree and 1-2+ years of experience (biotech/pharma industry preferred)
• Experience culturing a variety of cell types, including immortalized cell lines, primary cells and/or iPSC-derived cells
• Molecular biology techniques, including qRT-PCR and Western blotting
• Ability to optimize and troubleshoot molecular biology assays
• Technical versatility, with the ability to quickly learn and own a variety of assays as needed
• Strong attention to detail with excellent data organization and recordkeeping
• Able to follow SOPs and work independently in a high-throughput setting

Preferred Qualifications

• Familiarity or experience with oligonucleotide screening
• Flow cytometry and/or imaging experience