Associate Scientist/Scientist, mRNA Process Development

Key Responsibilities

• Develop processes for the synthesis of bacterial plasmid DNA, cell-free DNA and in-vitro transcribed (IVT) mRNA for different RNA therapeutic modalities (circRNA, saRNA, Linear mRNA)
• Develop purification methods (chromatography, tangential flow filtration) for plasmid DNA, cell-free DNA and mRNA
• Develop DNA and mRNA characterization tools including, but not limited, to capillary gel electrophoresis (CGE), HPLC, ELISA, cell-based protein expression, UV Spectroscopy and fluorescence assays
• Maintain accurate records using LIMS/ELN systems to support data integrity and reproducibility
• Prepare technical reports, presentations, and documentation to support internal decision-making and external regulatory submissions
• Support tech transfer activities to external CDMOs, including preparation of reports, training, and troubleshooting support
• Support regulatory submissions by generating reports and contributing to CMC documentation

Qualifications & Experience

• BS or MS degree in Biochemistry, Molecular Biology, Biochemical Engineering, Pharmaceutical Sciences, or a related field
• Minimum of 4+ (with MS degree) or 7+ (with BS degree) years of experience in the field related to RNA Biochemistry or Pharmaceutical Sciences
• Industry experience preferred but not required
• Knowledgeable and skilled in designing and conducting ex-vivo cell-based assays
• Demonstrated hands-on experience in ELISA, Western Blot, SDS-PAGE, standard molecular biology and RNA biochemistry tools (primer design, cloning, RT-PCR, PCR, DNA/RNA extraction and purification)
• Knowledge on nucleic acids purification tools (AEX, HIC & Affinity Chromatography, IP-RP HPLC) preferred
• Ability to use SnapGene, GraphPad Prism and Excel
• Strong problem-solving skills and decision-making skills with the ability to troubleshoot experimental processes and provide analysis
• Comfortable with fast-changing environment and flexible to effectively deliver results within reasonably established timelines
• Ability to work independently and collaboratively in a cross-functional, fast-paced multidisciplinary environment

This position is based in Singapore. Successful candidates must be willing to relocate to Singapore.