Technical Research Assistant I

We need to output HTML fragment with sections for headings: About the role, Responsibilities, Requirements, Qualifications, Skills, Benefits, Pay, Schedule. Only include sections that have real specific content. We must not repeat job title. Must not include Job ID etc. Must preserve all substantive info, not invent or summarize away detail. Remove boilerplate, navigation, legal, EEO, duplicated lines, application instructions. We need to parse the posting. Let's extract relevant content: - Intro about site: "Site: The Brigham and Women's Hospital, Inc. Mass General Brigham relies on a wide range of professionals..." This is generic description; maybe part of About the role? Could be considered "About the role" or "About the organization". The instruction: use

for section headings (e.g. About the role, Responsibilities, Requirements, Qualifications, Skills, Benefits, Pay, Schedule). So we can have About the role section containing description of the role and mission. - Job Summary: "Join the Santagata Lab at Brigham and Women’s Hospital (BWH) as a Technical Research Assistant I working on a multidisciplinary program to develop new targeted therapies for rare pediatric and adult brain tumors. We are seeking a talented and highly motivated individual to help identify and validate therapeutically actionable cell-surface proteins in rare brain cancers, with particular emphasis on targets for antibody-drug conjugates (ADCs) and other emerging targeted therapies. The position is supported by a Department of Defense research program focused on overcoming the major challenges posed by rare brain tumors: limited tissue availability, biological heterogeneity, and the lack of effective targeted treatments. The successful candidate will work with advanced tissue-imaging and spatial molecular technologies to quantitatively characterize therapeutic targets in patient tumor specimens and patient-derived models. Major approaches include cyclic immunofluorescence (CyCIF), ORION whole-slide multiplexed imaging, conventional immunohistochemistry and immunofluorescence, and integration with spatial transcriptomic data. The research program spans rare pediatric and adult brain tumors, including ependymoma, craniopharyngioma, atypical teratoid/rhabdoid tumor, pediatric high-grade glioma, rare meningiomas, embryonal tumors, and other molecularly defined tumors. A major goal is to determine whether drugs developed for more common cancers can be repurposed for rare brain tumors based on quantitative measurement of therapeutic target expression. You will be based in Dr. Sandro Santagata’s laboratory at BWH and work closely with investigators at Harvard Medical School’s Laboratory of Systems Pharmacology, Dana-Farber Cancer Institute, Boston Children’s Hospital, and collaborating laboratories. The project brings together neuropathologists, cancer biologists, chemists, imaging scientists, computational biologists, and neuro-oncologists. This is an excellent opportunity for an early-career scientist interested in translational cancer research, molecular imaging, biological chemistry, quantitative pathology, and therapeutic development. The position provides extensive hands-on experience with high-dimensional tissue imaging, antibody qualification, assay development, image analysis, and integration of molecular and morphologic data from human tumors." That's a lot of substantive content. Could be placed under About the role or Responsibilities? The "Job Summary" is like overview. The "PRINCIPAL DUTIES AND RESPONSIBILITIES:" list is a big list of duties. So we can have: - About the role: maybe the introductory paragraph about the lab and mission? Or we could put the Job Summary there. - Responsibilities: the list under PRINCIPAL DUTIES AND RESPONSIBILITIES. - Qualifications: the "Qualifications" section after duties includes bullet-like paragraphs: Bachelor’s degree..., Demonstrated interest..., Prior laboratory research experience..., Strong organizational skills..., Willingness to commit... - Skills/Abilities/Competencies Required: list of abilities. - Pay Range: "$21.00 - $29.01/Hourly Grade 5". Also mention benefits: "In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs". Also mention "Scheduled Weekly Hours 40", "Work Shift Day". So we have Pay, Benefits, Schedule sections. - Remote Type: Onsite, Work Location: 60 Fenwood Road. Could be part of About the role or maybe a separate section? Not in list of headings; we could include under About the role or maybe not needed. The instruction: only include sections when posting has real specific content. We have location info; could be placed in About the role as a sentence. - Employee Type: Regular. Could be part of About the role. We must not include boilerplate like EEO statement, Mass General Brigham Competency Framework, etc. We must not include duplicated lines. Ensure we preserve all substantive info. We need to output only HTML fragment, no markdown fences. We'll structure:

About the role

... (including site description, job summary, location, employee type, work schedule maybe? but schedule separate)

Responsibilities

• ... each duty

Qualifications

• ... each qualification bullet

Skills

• ... each skill bullet

Pay

$21.00 - $29.01 per hour (Grade 5)

Benefits

Comprehensive benefits package including career advancement opportunities, differentials, premiums, bonuses, and recognition programs.

Schedule

40 hours per week, day shift, onsite at 60 Fenwood Road.

We need to ensure we don't invent or summarize away detail. For responsibilities list, we must preserve all duties exactly as given, not omit any. The list is long; we need to include each duty as a separate

. Must not add extra info not present. Let's extract duties exactly as they appear after "PRINCIPAL DUTIES AND RESPONSIBILITIES:" until before "Qualifications". The text: "Develop a spatial molecular atlas of therapeutic targets in rare pediatric and adult brain tumors by generating, organizing, and quantitatively analyzing multiplexed tissue-imaging data across tumor types, cases, and histologic regions. Perform multiplexed tissue-imaging experiments using CyCIF, ORION, immunofluorescence, and related methods to quantitatively characterize cell-surface therapeutic targets in rare pediatric and adult brain tumors. Develop and qualify antibodies against therapeutic targets, including established and emerging ADC targets. Compare antibody clones and optimize staining conditions with attention to specificity, cellular localization, signal-to-background ratio, dynamic range, and reproducibility. Design and optimize multiplex antibody panels incorporating therapeutic targets together with tumor-lineage, proliferation, immune, vascular, and cell-state markers. Prepare and analyze human FFPE tissue specimens, tissue microarrays, and patient-derived tumor models while using tissue-sparing experimental strategies appropriate for scarce clinical samples. Perform quantitative analysis of whole-slide multiplexed images, including image quality control, single-cell segmentation and quantification, evaluation of target-expression heterogeneity, and visualization of spatial patterns. Integrate protein-imaging results with complementary molecular datasets, including spatial transcriptomic data, to identify and prioritize candidate therapeutic targets. Assist with studies comparing patient tumors with patient-derived cell lines and xenograft models to identify models that preserve clinically relevant therapeutic-target expression. Support development of standardized ORION multiplexed imaging assays designed to measure multiple therapeutic targets from a single tissue section and facilitate future clinical-trial and diagnostic applications. Maintain detailed experimental records and organized research datasets, including antibody-validation results, imaging experiments, sample metadata, and quality-control information. Troubleshoot and optimize experimental protocols and contribute ideas for improving assay performance, reproducibility, throughput, and tissue utilization. Prepare figures and datasets and contribute to scientific presentations, manuscripts, and collaborative project reports. Present work at laboratory and multidisciplinary project meetings and participate actively in discussions of experimental results and next steps. Support routine laboratory activities, including reagent preparation, ordering, equipment maintenance, sample organization, and adherence to laboratory safety procedures. Work collaboratively with scientists, physicians, computational researchers, and other research staff across BWH, HMS, Dana-Farber Cancer Institute, Boston Children’s Hospital, and collaborating institutions." We need to ensure each duty is a separate

. Some sentences have multiple sentences combined with periods; but each line appears separated by line breaks. We'll treat each line as a duty. Now qualifications: after "Qualifications" heading, we have: "Bachelor’s degree in biology, biochemistry, biological chemistry, chemical biology, biomedical engineering, biophysics, computational biology, bioinformatics, or a related biomedical or quantitative field. Demonstrated interest in biomedical research, cancer biology, molecular measurement, tissue imaging, or quantitative analysis of biological data. Prior laboratory research experience, particularly involving microscopy, immunofluorescence, molecular or biochemical assays, or quantitative data analysis, is preferred. Strong organizational skills and attention to detail, including the ability to work with complex sample sets and contribute to development of well-annotated research datasets. Willingness to commit to approximately two years in the position and grow within a collaborative, multidisciplinary research environment is strongly preferred." We'll make each a

. Skills/Abilities/Competencies Required: "Ability to perform reproducible experiments and maintain well-organized experimental records. Experience with or strong interest in immunofluorescence, immunohistochemistry, microscopy, protein biochemistry, molecular biology, or related experimental methods. Understanding of basic protein biochemistry and molecular interactions, including antibody–antigen binding, is desirable. Interest in quantitative analysis of biological data and willingness to learn image-analysis and computational tools. Familiarity with ImageJ/Fiji, Python, R, MATLAB, or related quantitative tools is desirable but not required. Ability to learn and troubleshoot technically sophisticated experimental workflows. Strong organizational, communication, and interpersonal skills. Ability to work both independently and collaboratively in a multidisciplinary research environment. Intellectual curiosity and enthusiasm for connecting experimental observations with questions in cancer biology and therapeutic development." Make each a

. Pay: "$21.00 - $29.01/Hourly Grade 5". Also note "At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package." This is more detail about pay determination. Should we include? It's substantive about pay. The instruction: Preserve ALL substantive information; do NOT invent or summarize away detail. So we should include that explanation as part of Pay section. However we must not create a Pay section unless posting actually states them. It does state pay range and explanation. So we can include a

with that explanation plus the range. Benefits: "In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth." Also earlier: "We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham." That's more generic. The benefits statement is specific enough. We'll include that. Schedule: "Scheduled Weekly Hours 40", "Employee Type Regular", "Work Shift Day (United States of America)", "Remote Type Onsite", "Work Location 60 Fenwood Road". We'll combine: 40 hours per week, day shift, onsite at 60 Fenwood Road. Also "Employee Type Regular" maybe not needed but could be included. However schedule section should include hours and shift. We'll mention regular full-time. Now About the role: we need to include site description and job summary. Let's combine: Site description: "Site: The Brigham and Women's Hospital, Inc. Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and