Senior Research Associate, in vivo Pharmacology

Key Responsibilities

• Work cross-functionally with scientists to help design and execute complex in vivo rodent studies to advance Stoke’s preclinical pipeline using antisense oligonucleotides to restore protein expression.
• Perform hands-on rodent procedures including bolus ICV and IT injections in both neonate and adult mice and rats. Other routes of injections include subcutaneous and intravenous injections with subsequent blood collection, serial sampling, and tissue collections.
• Execute dose-response, time-course, PK/PD, tolerability and efficacy studies.
• Maintain breeding colonies, wean and genotype, and utilize Softmouse to help expand colonies for experiments.
• Assess animal phenotypes by implementing various behavioral assays like Open Field, DigiGait, and Rotarod.
• Prepare tubes for sample collections and perform necropsies and subsequent tissue homogenization and molecular biology assays.
• Help analyze and interpret experimental data using appropriate statistical approaches and software.
• Maintain accurate laboratory records, electronic notebooks, study protocols, and data reports.
• Other tasks as assigned.

  Required Skills & Experience

  • S. with 4 years of experience or M.S. with 2 years of experience (relevant industry experience is a plus).
  • Degrees in Pharmacology, Neuroscience or relevant disciplines highly preferred.
  • Proficient in various routes of dose administration in mice and rats including, but not limited to, SC, IV, IT and ICV.
  • Experience with PK/PD studies in rodents.
  • Serial sampling in rodents is a plus.
  • Extensive knowledge of rodent anatomy to perform large scale necropsies.
  • Experience with anesthesia and pre- and post-op monitoring.
  • Skilled in surgical techniques such as stereotaxic ICV injections.
  • Knowledge with rodent behavioral studies of the CNS.
  • Experience with breeding and colony management.
  • Tissue processing and homogenization.
  • qPCR, ELISA, MSF.
  • Familiarity with Softmouse colony management Software is a plus.
  • Proficient with Excel, GraphPad Prism, and PowerPoint for data interpretation and presentation.
  • Electronic Notebook.
  • Familiarity with writing technical reports.
  • Strong aptitude for adapting technological advances in the field.