Senior Lab Research Technician

About the role

The Senior Lab Research Technician provides senior-level technical assistance and performs routine laboratory activities for a translational medicine laboratory studying how body composition changes, including muscle and fat loss, impact cancer prevention and survival. The position reports directly to the Principal Investigator, may require a probationary period, is contingent on continued funding, is ineligible for telework, and requires a background check.

Responsibilities

• Assisting with the establishment of a new research laboratory
• Maintenance of a mouse colony (weaning, genotyping, breeding, maintaining records)
• Planning and executing animal and cell culture experiments
• Processing of murine and human blood and tissue samples
• Routine laboratory housekeeping activities
• Maintenance of two IACUC protocols and IBC compliance
• Provides oversight and management of scientific research problems designed to understand mechanisms of cancer-associated wasting during early disease for future development of anti-cancer and anti-weight loss therapies
• Perform laboratory assays focused on anatomic, functional, and biochemical characterization of muscle, adipose tissue, and tumor
• Compile and analyze data from these assays
• Exercise judgment in prioritizing work to meet the objectives and deadlines associated with generating data for grant renewals, grant proposals, and submission or revision of manuscripts
• Provide high-level technical expertise in the design, performance, and troubleshooting of complex laboratory experiments
• Analyze, interpret, and manage experimental data
• Collaborate with the laboratory's Principal Investigator (PI) in developing projects for laboratory personnel and provide functional supervision and training of laboratory personnel
• Maintain compliance with regulatory agencies for the laboratory, including lab personnel safety, chemical safety, biosafety, IACUC, and IBC
• Serve as a resource for other laboratory members, including technicians, graduate students, undergraduates, and/or postdocs, in experimental design, evaluating research methodologies, and interpreting data
• Work independently with minimal supervision from the PI

Requirements

• Bachelor's degree in a related field and five years related laboratory research experience required
• Experience with in vivo animal research, molecular biology, and cell culture techniques
• Knowledge of physiology and metabolism
• Proficiency with the procedures required for successfully conducting the protocols carried out in lab
• Experience with quantitative RT‑PCR, Western Blot, ELISAs, and database management
• Knowledge of statistics, as applied to biomedical research
• Experience working with mice (including administration of substances, euthanasia, and tissue harvest), managing genetically modified mouse colonies, and genotyping
• Proficiency with spreadsheets and database applications
• Effective writing and organizational skills

Qualifications

• Experience with Microsoft Office, Excel, PowerPoint, GraphPad Prism, UVM Click, and VOS
• Experience in cancer, energy balance, or nutrition-related research

Pay

Anticipated Pay Range: $28.00 - $32.00/hour

Schedule