Scientist, Solid Tumor CAR-T

Key Responsibilities

• Design and execute in vitro CAR-T functional assays.
• Perform comprehensive phenotypic analysis of T cell differentiation, activation, and memory subsets.
• Evaluate CAR-T cell trafficking and tumor infiltration in vivo using flow cytometry and immunohistochemistry.
• Handling and engineering human and murine T cells as well as various cancer cells.
• Engineering and maintaining various cancer cell lines for model development.
• Analyze and interpret data, troubleshooting, maintain detailed experimental records.

Requirements

• A scientist in Immunology, Immuno-Oncology, Tumor Immunology, Cancer Biology, or a closely related field.
• Candidates completing their PhD within the past 3 years are strongly encouraged to apply.
• Primary human T cell isolation, activation, lentiviral transduction, and expansion is required.
• Experience with multicolor flow cytometry: panel design, data acquisition (e.g., Attune, Novocyte, Cytek Aurora), and analysis (FlowJo).
• Standard molecular biology: PCR/qPCR, lentiviral vector packaging and titration, Western blot.
• Experience with humanized mouse models for in vivo CAR-T evaluation.
• Familiarity with gene editing tools (CRISPR/Cas9) for cell engineering.
• Experience with in vitro Organoid cancer model establishment and expansion.
• Experience in solid tumor indications (e.g., lung, gastric, or other refractory cancers).
• Contributions to innovation in cell therapy (publications, patents, or conference presentations).