Scientist Bioscience
The Fountain Group · Gaithersburg, MD · 1 mo ago
On-siteOTHR$50/hrContract
About the role
Location: Gaithersburg, MD. Pay Rate: $50.00/hr. Duration: 2.5 months. Support upstream process development for microbial glycoprotein production and high-throughput screening.
Responsibilities
- Support upstream process development for microbial glycoprotein production and high-throughput screening.
- Execute microbial colony screening workflows using automated liquid-handling systems.
- Prepare microbial cultures, reagents, samples, plates, and materials for automated processing.
- Perform high-throughput assays, including ELISA and other plate-based immunoassays.
- Execute molecular biology workflows including DNA extraction, PCR, plasmid preparation, transformation, and agarose gel electrophoresis.
- Support mutant library screening and evaluation of microbial colonies for enhanced glycoprotein expression.
- Support sample preparation for whole-genome sequencing and experiments involving enzymatic glycosylation.
- Analyze assay data, identify experimental trends, and document results in electronic laboratory systems.
- Follow approved laboratory, biosafety, quality, and data-integrity procedures.
Requirements
- Master's or PhD in Molecular Biology, Biochemistry, Microbiology, Biotechnology, Bioengineering, or a related scientific discipline preferred.
- Bachelor's degree with relevant hands-on laboratory experience may be considered.
Skills
Required:
- Molecular biology techniques
- Microbial culture and bacterial expression systems
- ELISA / immunoassays
- PCR, DNA extraction, plasmid preparation, transformation, agarose gel electrophoresis
- High-throughput microbial screening
- Recombinant protein/glycoprotein production
- Laboratory data analysis and experimental documentation
Preferred:
- Bacterial fermentation
- Automated liquid-handling platforms
- Laboratory automation / robotic workflows
- Bacterial strain engineering and mutant library generation
- Colony picking and automated sample processing
- Whole-genome sequencing sample preparation
- Enzymatic glycosylation / glycoengineering
- Glycoprotein characterization
- Excel, GraphPad Prism, or equivalent data-analysis tools
- Biotechnology, pharmaceutical, or vaccine-development laboratory experience
Pay
$50.00/hr