Scientist Bioscience

Description Seeking a highly motivated and talented scientist to join our Chemical Biology and Proteomics group within Discovery Sciences. You will work as an integral member of a cross-functional team to interrogate mechanisms of action for therapeutic agents, and to propose and validate biological hypotheses for agents within the cardiovascular, renal and metabolism (CVRM) area. Similar Workflows to Associate Scientist: Mammalian cell culture, protein conjugation, purification, and UV-Vis spectroscopy characterization.Affinity Enrichment Proteomics & Mass Spec: Carrying out advanced mass spectrometry-based proteomics with affinity enrichments.Proximity Ligation Assays: Development of proximity ligation assays (highlighted as a preferred / nice-to-have skill, not an absolute requirement).Level of Autonomy: Expected to operate with significantly higher independence than the Associate Scientist.Design and execute discovery proteomic assays using mass spectrometry to support mechanistic studies, discover novel targets, and elucidate biological regulators of disease biology.Perform proximity biotinylation studies in cells, using established automated affinity purification workflows, and data independent acquisition (DIA) mass spectrometry methods.Perform target validation by developing proximity ligation assays (PLA) and cell-based assays to assess protein-protein interactions using chemical tools and confocal microscopy.Generate protein conjugates and characterize protein labeling by UV-Vis spectroscopy.Analyze and interpret experimental data using established bioinformatic pipelines.Maintain detailed and accurate electronic laboratory notebooks.Represent CBP team, and communicate with project discipline leads, and contribute to generation of mechanistic hypothesis through interpretation of proteomics data. Required Qualifications Hands-on proteomics experience is mandatory (from graduate, postdoctoral, or industry work), alongside mammalian cell culture and data analysis.Education & Experience Options:PhD with proteomics experience from graduate research or postdoc work, ORMaster's Degree with prior industry proteomics experience (minimum 1 year industry proteomics, 3-5 years hands-on lab experience).Knowledge and hands-on experience with proteomic sample preparation.Experience in mammalian cell culture.Experience employing cell-based and proteomic assays to understand small molecule mechanisms of action.Demonstrated ability to work effectively both independently and collaboratively, contribute to scientific discussions, solve problems, and deliver high-quality results within agreed timelines.Experience developing or applying proximity ligation assays or related proximity-based protein-protein interaction validation assays.Experience using LC-MS/MS platforms and semi-automated instrumentation.Experience using proteomics search and analysis tools, such as Spectronaut, Proteome Discoverer, MaxQuant, Skyline and Xcalibur. Benefits Healthcare Insurance: Synectics offers eligible employees and their dependents healthcare coverage through BlueCross BlueShield of Illinois. Eligibility begins on the 1st day of the calendar month following 60 days of continuous full time employment with Synectics. Premiums are subsidized by Synectics. Dental Insurance: Synectics offers eligible employees and their dependents a dental plan through MetLife. Eligibility begins on the 1st day of the calendar month following 60 days of continuous full time employment with Synectics. Vision Insurance: Synectics offers eligible employees vision insurance through VSP. Eligibility begins on the 1st day of the calendar month following 60 days of continuous full time employment with Synectics. 401(k) Plan: The Synectics Inc. Investment Savings Retirement Plan. Synectics offers all employees who are 21 years of age or older the opportunity to invest in the 401(k) Plan on the first of the month following at least 1 calendar year after employment begins. Synectics is an equal opportunity employer.