Roth Lab Laboratory Research Technician III

Essential Functions

• Human cell culture (routine handling, transfection, flow cytometry).
• Manipulating and screening libraries of bacterial and yeast cells.
• DNA isolation from cells, PCR amplification, and gel purification of libraries.
• Preparing and processing of DNA libraries for sequencing using high throughput sequencing platforms (e.g., Illumina and PacBio).
• Operating liquid- and plate-handling robotics.
• Maintaining laboratory stocks of media supplies, reagents, buffers, plates, etc.
• Performing routine maintenance of laboratory instruments.
• Assisting in analyzing and summarizing data for scientific communications (e.g., grant applications, peer-reviewed publications).
• Maintaining records of experimental data and communicating results to lab members.
• Reviewing pertinent scientific literature.

Qualifications

• Bachelor’s degree with 2 years relevant work experience or equivalent experience pursuing a Master’s degree.
• Understanding of basic molecular biology and genetics.
• Excellent verbal and written communication skills.
• Theoretical and practical knowledge of molecular cloning techniques (DNA/RNA isolation, restriction digestion, PCR amplification, purification, etc.).
• Experience with human cell culture OR the handling/manipulation of yeast.
• Experience with the proper handling and genetic manipulation of bacteria.
• Proficiency with basic computational tools used in biology (Excel, BLAST, primer design software).
• Experience with the preparation and quality control of sequencing libraries (such as Illumina or PacBio) would be an asset.
• Experience with flow cytometry and FACS would be an asset.

Physical Effort

• Physical effort associated with wet lab work, i.e. standing while conducting experiments, sitting while working on computer, walking back and forth in the lab to sinks and microscopes, lifting packages less than 20lbs., etc.

Work Schedule

Monday - Friday, 8:30 a.m. - 5:00 p.m.

Work Arrangement

On-Campus: Teams that work on campus, in an office, or in a lab.