Research Technician III

About the role

Duke University School of Medicine is the youngest of the nation's top medical schools, ranked sixth among medical schools nationally. The David Lab at Duke University develops and uses new DNA sequencing tools to study diet and the gut microbiome, focusing on objective measures of food intake through plant and animal species identification via DNA. Projects span North Carolina and global collaborations, supported by funding from Schmidt Sciences, the Chan Zuckerberg Initiative, NIH, the Gerber Foundation, and the Burroughs Welcome Fund.

Responsibilities

• Sample Intake and Chain-of-Custody: Receive and process wastewater samples, mail-in citizen-science kits, and collaborator samples; verify metadata, barcodes, temperature, and control logs. Accession, aliquot, and store samples with appropriate protocols and maintain accurate lab records.
• Molecular Workflows and QC: Perform DNA extractions from concentrates, swabs, and solids; quantify nucleic acids; prepare amplicon libraries (e.g., trnL, 12SV5) and other targeted assays with accuracy and consistency. Set up and run PCR and related workflows using standard instruments (thermocyclers, fluorometers, plate readers), controls, and standards. Prepare sequencing libraries and support sequencing workflows, including coordination with internal or external sequencing resources. Troubleshoot assays, identify sources of technical variation, make minor protocol modifications, and document deviations and corrective actions. Summarize quality-control metrics and experimental results for project tracking, reporting, and downstream analysis.
• Lab Operations: Prepare buffers, reagents, and lab materials; track lot numbers and manage inventory; place orders and receive supplies. Calibrate and maintain common equipment; coordinate preventative maintenance and service calls. Operate and maintain laboratory robotics and automated liquid handling systems. Uphold biosafety, chemical hygiene, and waste-disposal procedures. Ensure temperature monitoring in freezers and fridges. Maintain and improve standard operating procedures and protocols. Conduct routine, detail-intensive experiments with enthusiasm, seeking ways to improve speed and efficiency without compromising quality.
• Data Collection and Processing: Assist in collecting, processing, and managing data from genomic experiments. Assemble and conduct quality assurance of participant stool collection kits and wastewater collection kits. Manage shipments and returns of samples and ensure timely processing. Maintain accurate sample and laboratory records, including metadata, QC results, freezer inventories, and chain-of-custody documentation. Organize raw experimental data and laboratory outputs for handoff to senior lab staff and downstream computational analysis.
• Collaboration and Teamwork: Coordinate with field sampling teams on field sampling needs, sample readiness, and turnaround. Train and help oversee student assistants and other junior personnel on core laboratory workflows, safety procedures, documentation standards, quality expectations, and timelines. Work collaboratively within the lab team and contribute to a positive and productive research environment.

Minimum Requirements

• Bachelor's degree in botany, biology, zoology, psychology, or a related scientific field.
• Two years of research experience.

Preferred Qualifications

• Experience with DNA extraction, PCR, sequencing-library preparation, and/or laboratory automation.
• Prior experience with robotic systems.

Skills

• Technical Proficiency: Experience in genomics or related fields, with a background in DNA extraction, PCR, and/or sequencing.
• Growth Mindset: Strong willingness to learn, adapt, and develop new skills; curiosity and proactive problem-solving.
• Dedication and Efficiency: Passion for detail-oriented experimental work, self-motivation, and commitment to continuous improvement.
• Communication Skills: Ability to communicate effectively within a team and contribute to a collaborative research environment.

Pay

Duke University provides an annual base salary range of USD $22.61 to USD $34.27 per hour. Salary decisions consider scope and responsibilities, candidate experience, education/training, internal peer equity, market and organizational considerations.