Principal Associate Scientist, Target Validation

About the role

The Research Department at Alnylam is seeking an experienced, highly motivated Principal Associate Scientist to validate therapeutic gene targets through in vitro and in vivo mechanistic studies to support discovery efforts for future RNAi medicines.

Responsibilities

• Engage in a variety of investigative, mechanistic target validation and proof-of-concept preclinical research across various tissues and therapeutic areas of interest
• Perform in vivo proof-of-concept studies to evaluate novel extrahepatic, targeted siRNA delivery platforms across multiple pipeline programs and early discovery
• Develop, optimize, and validate new assays to support emerging targets, biomarker development and tissue-specific delivery evaluation
• Contribute to internal decision-making by integrating knockdown data with orthogonal readouts including protein, functional, and transcriptomic endpoints
• Prepare clearly articulated experimental reports and data presentations for a variety of audiences
• Maintain detailed, accurate laboratory notebooks and electronic data records with meticulous attention to data integrity

Qualifications

• BS/MS in Biology, Cell/Molecular Biology or related field with 5-8 years of work experience, preferably in an industrial setting
• Prior experience conducting and interpreting in vivo rodent studies, including subcutaneous, IP and IV dosing, retro-orbital bleeding, and tissue dissections
• Proficiency in RNA extraction across diverse tissue types, and molecular biology assays for RNA/DNA/protein quantification (qPCR, Western blotting, ELISA, immunoprecipitations)
• Hands-on experience in mammalian cell culture including maintenance, transfection, and work with primary cells (e.g., primary hepatocytes, cardiomyocytes, muscle cells)
• Strong quantitative data analysis skills; proficiency with GraphPad Prism, Excel, and standard statistical methods; meticulous laboratory notebook documentation
• Familiarity with muscle, cardiovascular or endocrine disease biology and relevant preclinical models preferred
• Excellent lab technique and demonstrated ability to perform and troubleshoot assays independently or with limited direction of supervisor(s)/project leader(s)
• Strong interpersonal skills and excellent communication skills (written and oral) are required

U.S. Pay Range

$95,600.00 - $143,400.00

Benefits

• Comprehensive benefits including medical, dental, and vision coverage
• Life and disability insurance
• A lifestyle reimbursement program
• Flexible spending and health savings accounts
• A 401(k) with a generous company match

Learn more about these and additional benefits offered by Alnylam by visiting the Benefits section of the Careers website: https://www.alnylam.com/careers