Laboratory Research Technician IV

About the role

The Pulmonary, Allergy, Critical Care and Sleep Medicine Division of the DOM is seeking a Laboratory Technician to join our team. This position is based at multiple locations including Bridgeside Point, BST, UPMC Hospitals, and more.

Responsibilities

• Performs DNA and RNA isolation, genotyping analysis, protein extraction, and multiplex immunoassay platforms.
• Conducts gene promoter activity analysis, cell culture, plasmid transfection, virus transduction, and basic cellular function measurements.
• Generates genetically modified cell lines using CRISPR-Cas9 system.
• Utilizes multiplex immunoassay platforms (including MSD, Bio-Plex) for biomarker and cytokine analysis.
• Performs machine maintenance procedures such as calibration and validation.
• Writes manuscripts and grant proposals and prepares data visualizations.
• Maintains inventory, orders, and logs.
• Collaborates with other researchers and staff regarding laboratory procedures and experiments.
• Works closely with the Lab Manager for assay development and protocol.

Requirements

• Bachelor's Degree in a related field.
• 3+ years of relevant experience in a laboratory setting.
• Familiarity with TECAN.
• Experience with DNA and RNA isolation, genotyping analysis, protein extraction, and multiplex immunoassay platforms.
• Basic cell culture and co-culture, plasmid transfection, virus transduction, and basic cellular function measurements.
• Experience with gene promoter activity analysis using luciferase reporter system.
• Experience with CRISPR-Cas9 system for generating genetically modified cell lines.
• Physical ability to sit/stand for up to 8 hours a day.

Qualifications

• Must be able to sit/stand for up to 8 hours a day.
• Must be familiar with TECAN.
• Must have experience with DNA and RNA isolation, genotyping analysis, protein extraction, and multiplex immunoassay platforms.
• Must have experience with gene promoter activity analysis using luciferase reporter system.
• Must have experience with CRISPR-Cas9 system for generating genetically modified cell lines.

Skills

• Strong analytical and problem-solving skills.
• Excellent communication and collaboration skills.
• Proficiency in laboratory equipment and techniques.
• Ability to work independently and as part of a team.

Benefits

Details about benefits are not specified in the job posting.

Pay

Details about pay are not specified in the job posting.

Schedule

Details about schedule are not specified in the job posting.

Additional Information

The University of Pittsburgh is an equal opportunity employer / disability / veteran. The assignment category is Full-time regular. The job classification is Staff. The laboratory research technician IV job family is Research. The work schedule is Monday - Friday; 8am - 4:30pm. The work arrangement is On-Campus: Teams that work on campus, in an office, or in a lab.