Biochemist I (Must Have Tissue Culture, Cell Culture & Centrifugation)

Position

Position : Biochemist I (Must Have Tissue Culture, Cell Culture & Centrifugation)

About the Role

The Biochemist I is expected to work independently, perform all laboratory functions that involve Tissue Culture such as Hybridoma roller bottle cell culture, ascites production, insect cell shaker culture, recombinant protein production, in-process assays, and safety procedures. The position is expected to implement and follow all new and current department policies and procedures per departmental SOP's and Batch Records for all manufacturing functions within the department.

Responsibilities

• Responsible for learning the sterile Tissue Culture technique to independently work under Biosafety hood.
• Follow the Tissue Culture SOP to make different kinds of monoclonal antibody and cell related products by Hybridoma roller bottle cell culture and ascites production and other mammalian cell related products.
• Follow the Tissue Culture SOP to perform insect cell shaker bioreactor for producing recombinant protein by using Baculovirus or E.coli expression system.
• Complete Batch Records accurately and in a timely manner.
• Follow all SOP's and Work Instructions precisely.
• Perform clarification and concentration of Tissue Culture supernatant using the Tangential Flow system and /or centrifugation.
• Perform in-process assays Nephelometer, Mycoalert and isotype ELISA.
• Help maintain the cell bank inventory by accurate retrieval and placement of vials from LN2 freezer.
• Promote a safe working environment. Follows client Environmental, Health and Safety (EH&S) policies and procedures.
• Takes responsibility for safety in immediate work area.
• Participates in EH&S programs. Notifies supervisors of all observed hazardous conditions or unsafe work practices.
• May provide recommendations on maintaining the safety of the work environment.

Qualifications

• Education: Typically requires BS degree at a 4-year degree program in science or equivalent combination of related education and experience.
• Work Experience: Typically requires 0-2 years' experience in a Biotech/ Life Science manufacturing environment. Have some working knowledge in the science disciplines and operation of basic lab equipment.

Techniques Required

• Pipetting
• Dilution
• Centrifugation
• Sterile technique

Techniques Desired

• Not specified

Knowledge and Skills Required

• Demonstrated ability to use laboratory instruments and applicable computer software.
• Knowledge of general laboratory safety.
• Effective interpersonal skills, verbal, and written communication.
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, complete, and legible records and logs.
• Possess analytical problem-solving skills.
• Must be able to lift a minimum of 25 pounds weight.

Physical Requirements

• General physical requirements: Sedentary work
• Visual acuity requirements: Inspection / Administrative
• Motion and sensory requirements: Talking, hearing, and walking.

Principal Accountabilities

• Implement and follow all new and current department policies and procedures per departmental SOP's and Batch Records for all manufacturing functions within the department.
• Follow all departmental guidelines and procedures with minimal Departmental Supervision.
• Maintain all manufacturing batch records.
• Be able to perform data entry in all required data bases.
• Other duties as assigned by Supervisor.