Biochemist I (Must Have Tissue culture, Cell Culture, Pipetting &Dilution)

Description

Position : Biochemist I (Must Have Tissue culture, Cell Culture, Pipetting &Dilution)
Location : San Diego, CA
Duration : 12 Months
Total Hours/week : 40.00
1st Shift
Client: Medical Device Company
Job Category Healthcare
Level Of Experience: Entry Level
Employment Type: Contract on W2 (Need US Citizens Or GC Holders Only)
Work hours: 8 to 5 with ½ hour lunch and 15 minutes break twice a day.

Techniques Required

• Pipetting
• Dilution
• Centrifugation
• Sterile technique

Techniques Desired

• Cell culture

Position Qualifications

• Education: Typically requires BS degree at a 4 year degree program in Science or equivalent combination of related education and experience.
• Work Experience: Typically requires 0-2 years' experience in a Biotech/ Life Science manufacturing environment. Have some working knowledge in the science disciplines and operation of basic lab equipment.
• Required Licenses: None

Knowledge and Skills Required

• Demonstrated ability to use laboratory instruments and applicable computer software.
• Knowledge of general laboratory safety
• Effective interpersonal skills, verbal and written communication
• Demonstrated ability to follow established policies and procedures.
• Demonstrated ability to keep neat, accurate, complete and legible records and logs.
• Possess analytical problem solving skills.
• Must be able to lift a minimum of 25 pounds weight.

Physical Requirements

• General physical requirements: Sedentary work
• Visual acuity requirements: Inspection / Administrative.
• Motion and sensory requirements: Talking, hearing and walking.

Principal Accountabilities

• Implement and follow all new and current department policies and procedures per departmental SOP's and Batch Records for all manufacturing functions within the department.
• Follow all departmental guidelines and procedures with minimal Departmental Supervision.
• Maintain all manufacturing batch records.
• Be able to perform data entry in all required data bases
• Other duties as assigned by Supervisor.

Responsibilities

• 60-80% Operations / 40-20% technical training
• Responsible for learning the sterile Tissue Culture technique to independently work under Biosafety hood.
• Follow the Tissue Culture SOP to make different kinds monoclonal antibody and cell related products by Hybridoma roller bottle cell culture and ascites production and other mammalian cell related products.
• Follow the Tissue Culture SOP to perform insect cell shaker bioreactor for producing recombinant protein by using Baculovirus or E.coli expression system.
• Complete Batch Records accurately and in a timely manner.
• Follow all SOP's and Work Instructions precisely
• Perform clarification and concentration of Tissue Culture supernatant using the Tangential Flow system and /or centrifugation.
• Perform in-process assays Nepphenomelter, Mycoalert and isotype ELISA.
• Help maintain the cell bank inventory by accurate retrieval and placement of vials from LN2 freezer.
• Promote a safe working environment. Follows Client Environmental, Health and Safety (EH&S) policies and procedures.
• Takes responsibility for safety in immediate work area.
• Participates in EH&S programs. Notifies supervisors of all observed hazardous conditions or unsafe work practices.
• May provide recommendations on maintaining the safety of the work environment.
• Perform other related duties and assignments as required.