Temporary Protein Expression Scientist
Overview
We are currently seeking a highly skilled Protein Expression & Purification Scientist (Contractor) to join a well-known client’s Discovery and Structural Biology team. This role will support cutting-edge drug discovery programs through molecular biology, protein expression, and purification workflows in a dynamic research environment.
Responsibilities
- Molecular Biology & Cloning
- Perform molecular biology workflows including cloning, vector design, construct optimization, and sequence verification.
- Execute PCR, primer design, and cloning strategies (restriction-based and recombination-based methods).
- Protein Expression
- Execute protein expression using:
- Baculovirus Expression Vector Systems (BEVS) in insect cells, including virus generation, amplification, and optimization.
- E. coli expression systems, including IPTG-induced and autoinduction methods.
- Congratulations! Small-scale expression screening to evaluate constructs and conditions.
- Protein Purification & Characterization
- Purify recombinant proteins using chromatographic techniques (affinity, ion exchange, size exclusion chromatography).
- Optimize expression and purification workflows to achieve required yield, purity, and quality.
- Perform analytical characterization including SDS-PAGE, Western blotting, SEC, and related assays.
- Data & Documentation
- Maintain accurate laboratory records in accordance with internal documentation standards (Benchling experience a plus).
- Communicate experimental results, observations, and recommendations clearly to team members.
- Compliance & Collaboration
- Adhere to laboratory safety policies and regulatory requirements.
- Collaborate with cross-functional teams to support research programs and project goals.
- Bachelor’s degree with 3+ years, or Master’s degree with 1+ year of relevant industry experience in biochemistry, molecular biology, structural biology, or related field.
- Strong expertise in molecular biology and cloning techniques.
- Demonstrated experience with protein expression in BEVS/insect systems and E. coli.
- Experience with high-throughput expression screening and optimization workflows.
- Proven experience with chromatographic purification methods and protein characterization.
- Preferred: Experience with biophysical characterization techniques, such as LC MS, SPR, MST, SEC MALS, nanoDSF, or mass photometry.
- Bachelor’s or Master’s degree in Biochemistry, Molecular Biology, Structural Biology, or a related scientific discipline required.
- Strong analytical and troubleshooting skills across cloning, expression, and purification workflows.
- Ability to work independently while collaborating effectively within cross-functional teams.
- Excellent organizational and documentation skills with attention to detail.
- Strong verbal and written communication skills.
- Ability to manage multiple priorities and meet timelines in a fast-paced research environment.
- Commitment to quality, reproducibility, and continuous improvement.
- Full-time employees are also eligible for benefits options such as health coverage, life insurance, disability insurance, and 401k benefits.
Experience
Education
Qualifications
Benefits
About the Company
Advanced Group is committed to providing employment opportunities without regard to sex, race, color, age, national origin, religion, gender identity or expression, sexual orientation or sexual preference, pregnancy or maternity, genetic information, marital status, disability, veteran status, or any other basis protected by applicable federal, state or local law.
Advanced Group complies with federal and state disability laws and makes reasonable accommodations for applicants and candidates with disabilities. If a reasonable accommodation is needed to participate in the job application or interview process, please contact accommodationrequest@advancedgroup.com.