Stem Cell Research Associate II - 141152
UC San Diego Health · San Diego, CA · Yesterday
Research$32.79–$52.75/hrFull-time
About the role
The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 50 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff).
Responsibilities
- Independently perform and oversee pre-clinical experiments and manage clinical samples designed for collection of safety and efficacy data of a gene therapy product.
- Work with various cell types, human blood and mouse models.
- Perform general lab duties.
- Be the EH&S biosafety contact.
- Equipment maintenance.
- New employee onboarding.
- Assist in mouse colony management.
- Lentivirus production.
- Cell culture.
Requirements
- Theoretical knowledge in cellular and molecular biology, or other closely related field typically acquired by a Bachelor's degree, or an equivalent combination of education and experience.
- Working knowledge and understanding of sterile techniques and working with mammalian cell culture practices and microscopy.
- Demonstrated experience performing repetitive and specialized laboratory experimental procedures and following experimental protocols.
- Experience operating, maintaining and repairing a variety of laboratory equipment.
- Effective and diplomatic oral and written communication and interpersonal skills.
- Excellent organization skills, sufficient to multi-task in an extremely fast-paced environment with constant changing priorities.
- Working knowledge of various computer software, hardware, and standard office sufficient to record scientific data and results, and prepare reports.
- Ability to quickly learn new laboratory procedures and adapt lessons from one learning episode to similar situations.
- Ability to maintain a high level of motivation to contribute to cutting edge research, and seek and learn new experimental methods as required.
- Demonstrated experience with mouse handling, preferably with blood sampling, tissue collection, and survival surgery.
- Demonstrated ability to follow established Safety and Biohazardous Material Guidelines.
- Knowledge of lentivirus production, virus transduction of mammalian cells, stem cell isolation, and GLPs is desirable.
Qualifications
- Demonstrated experience with molecular and cellular biology including: RNA/DNA isolation, Real-time PCR, tissue culture, stem cell culture and isolation, and genetic manipulations techniques such as CRISPR/Cas9-mediated technology.
- Demonstrated skill in the preparation of reagents and maintenance of laboratory stocks.
- Demonstrated ability to keep clear, detailed, accurate and organized data records.
- Demonstrated ability to work independently or as part of a research team, and interact effectively with collaborating labs and others in scientific community.
Skills
- Ability to use mathematical skills to analyze and develop statistical data.
Benefits
N/A
Pay
Annual Full Pay Range: $68,466 - $110,142 (will be prorated if the appointment percentage is less than 100%)
Hiring Pay Scale: $32.79 - $52.75
Schedule
Days, 8-Hour Shifts, Monday-Friday