SENIOR SCIENTIST, STRAIN ENGINEERING

Responsibilities

• Help develop Sutro’s prokaryotic protein expression platforms
• Molecular biology including cloning, PCR, generating gene knock-ins and knock-outs
• Screening of new strains for growth, protein expression and product quality in shake flasks
• Evaluate strain performance in small-scale bioreactors through fed-batch or continuous fermentation
• Conduct mid-stream processing including harvest, cell lysis, clarification, and filtration
• Strain banking activities including Development Cell Bank production
• Coincide with external collaborators, make presentations to internal and external collaborators and senior management
• Author internal and external publications

Qualifications

• Ph.D. in Biochemistry or a related discipline with at least 5 years’ experience
• Hands-on experience working with small-scale bioreactors is required
• Experience optimizing and evaluating prokaryotic protein expression strains using plasmid transformation, lambda-red recombination and shake flask or bioreactor overexpression preferred
• Experience working with in vivo protein expression required
• Experience working with antibodies or antibody fragments preferred
• Familiarity with molecular biology techniques including cloning, PCR, gene knock-ins and knock-outs
• Proficiency with common protein analytical techniques including SDS-PAGE, Western blotting and ELISA
• Familiarity with statistical analysis software for design of experiments, such as JMP, and sequence analysis software, such as SnapGene

Key Internal and External Collaborations and Interface

• Interface with DSP and analytical groups within Sutro
• Manage external collaborations with vendors such as Genscript or InfinixBio required for strain engineering activities