Senior Research Technician - Molecular Microbiology

About the role

The Kanjee lab studies how malaria parasites infect human red blood cells (RBCs). We are interested in identifying host receptors required by the parasite during RBC invasion, with a specific focus on the understudied parasite Plasmodium vivax. We use red blood cell genetics approaches (CRISPR/Cas9 knockout/knockdown & gene complementation) to understand the functional mechanisms of candidate host receptors during parasite invasion. This position in the Kanjee lab will assist with experiments involving general molecular cloning, designing CRISPR/Cas9 knockout, knockdown and host receptor gene complementation constructs, in vitro culturing of primary and immortalized erythroid cells, culturing of malaria parasites, validation of host RBC genetic knockouts/knockdowns/complementation via flow cytometry and Western blot. These experiments will involve: collection of data, preparation of cultures, media and set-up and maintenance of equipment.

Primary Duties & Responsibilities

• Performs complex analysis/projects according to research protocols, explaining methods and procedures to other technicians.
• Enters data and maintains current data files on research projects.
• Maintains consistency in data quality.
• Prepares tissue cultures, reagents, probe tests, etc.
• Performs preliminary statistical analysis of data.
• Affords technical support to technicians.
• Assists in the training and development of technicians, acting as a group leader.
• Performs other duties as assigned.

Working Conditions

Works in a laboratory environment with potential exposure to biological and chemical hazards. Must be physically able to wear protective equipment and to provide standard care to research animals.

Education

Required Qualifications: Master’s degree or combination of education and/or experience may substitute for minimum education.

Skills

Not Applicable

Preferred Qualifications

• Prior experience with in vitro cell culture in BSL2 work environment.
• Prior experience with mammalian genetics approaches and molecular cloning.
• Prior experience with parasite culture.
• Working knowledge of spreadsheets and statistical analyses.
• Excellent record keeping, organizational skills, and ability to interact with others.

Salary Range

$18.77 - $31.94 / Hourly

Benefits Statement

Personal Up to 22 days of vacation, 10 recognized holidays, and sick time. Competitive health insurance packages with priority appointments and lower copays/coinsurance. Take advantage of our free Metro transit U-Pass for eligible employees. WashU provides eligible employees with a defined contribution (403(b)) Retirement Savings Plan, which combines employee contributions and university contributions starting at 7%. Wellness Wellness challenges, annual health screenings, mental health resources, mindfulness programs and courses, employee assistance program (EAP), financial resources, access to dietitians, and more! Family We offer 4 weeks of caregiver leave to bond with your new child. Family care resources are also available for your continued childcare needs. Need adult care? We’ve got you covered. WashU covers the cost of tuition for you and your family, including dependent undergraduate-level college tuition up to 100% at WashU and 40% elsewhere after seven years with us.