Senior Associate Scientist, iSci_eDev

Key Responsibilities

• Support a variety of investigative, mechanistic siRNA research efforts to evaluate RNAi compounds by applying various biochemistry and cell/molecular biology assays.
• Maintain and manipulate diverse mammalian cell lines and primary cells.
• Perform transfection (lipid-based and electroporation) and free-uptake for siRNA delivery.
• Quantify siRNA/mRNA/protein using various assays (qPCR, Western blotting, ELISA, immunoprecipitations, imaging).
• Develop, validate, and troubleshoot cell-based assays (e.g., cell viability, reporter gene assays, and high-content imaging) to assess siRNA / monomer safety profiles.
• A willingness to learn new techniques to support in vitro and in vivo studies.
• Rigorously analyze complex datasets using software like GraphPad Prism or FlowJo etc.
• Present experimental results clearly to cross-functional project teams to influence drug design.

Qualifications

• BS/MS in Biology, Cell/Molecular Biology, Biochemistry or related field with 3-5 years of work experience preferably in an industrial setting, preferably in a field of oligonucleotides.
• Experience in mammalian cell culture and molecular biology techniques for RNA/DNA/protein quantification, ELISA, RT-qPCR and cell-based assays.
• Experience in Fluorescence microscopy (e.g., confocal, or live-cell imaging).
• Excellent lab technique and ability to work independently.
• Strong interpersonal and communication skills (written and oral) are required.
• Excellent organization, documentation, and record-keeping in ELN.
• Passionate about scientific discovery and eager to learn new skills and contribute to the success of the organization.

About Alnylam

We are the leader in RNAi therapeutics – a revolutionary approach with the potential to transform the lives of people with rare and common diseases. Built on Nobel Prize-winning science, Alnylam has delivered the breakthroughs that made RNAi therapeutics possible and are just at the beginning of what’s possible. Our deep pipeline, late-stage programs, and bold vision reflect our core values: fierce innovation, passion for excellence, purposeful urgency, open culture and commitment to people. We're proud to be a globally recognized top employer, where an authentic, inclusive culture and breakthrough thinking fuel one another.