Scientist II-Purification and Tissue Culture (Must Have Tissue Culture & Purification)

Position : Scientist II-Purification and Tissue Culture Location : Columbus, NE Duration : 12 Months Contract Total Hours/week : 40.00 pay rate:$20-30/hr Description We are seeking a highly motivated and organized Scientist II to contribute to the research and development of monoclonal antibodies in our Antibody Discovery team. This individual will be responsible for working in a dynamic team environment and be a contributor to multiple work streams, including but not limited to tissue culture and protein purification. The position requires not only extensive hands-on experience with a broad range of cell culture techniques but also expertise in protein purification and molecular biology, as well as an understanding of immunology. This individual will also be responsible for spearheading process improvements as well as the transfer of new processes to Operations. The successful candidate is expected to set up and execute experiments, be detail oriented, skilled at data analysis and troubleshooting and will be accountable for preparing and presenting data at lab meetings with guidance regarding required tasks and expected results. Duties And Responsibilities Responsible for maintenance, scale-up and characterization of hybridoma and other cell lines. Specific requirement for hands-on experience with aseptic technique and cell-culture techniques. Perform protein purification, including affinity chromatography as well as other related biochemical techniques including SDS-PAGE, Western Blot, UV-Vis spectrophotometry and FPLC. Assist with molecular biology and animal handling experiments. Day-to-day problem solving and cross-functional team-based activities to ensure that daily operations and timelines are met. Independently analyze own research data, solve problems with minimal guidance and effectively plan follow up experiments. Will be expected to efficiently prioritize individual workload. Involvement with scheduling, operational and maintenance duties required within the laboratory. Review and approval of data, drafting appropriate protocols, reports, batch records and process documents as required. Knowledge And Skills Proficient in tissue culture techniques; including experience growing and producing antibodies from hybridomas. Experience with protein purification, including various affinity chromatography and biochemical techniques. Prefer familiarity with FPLC and/or HPLC. Molecular biology and cloning. Animal handling. Assay automation. Preparation of documentation for process transfer to Operations. Flexible team player with good problem-solving and communication skills. Education And Experience A Bachelor’s or Master of Science degree in cellular or molecular biology, immunology or related scientific discipline and a minimum of 2-5 years of laboratory experience required. Tissue culture and/or purification experience required.