Scientist

About the role

We are seeking an experienced individual with cancer research and oncology drug discovery expertise to join the Discovery Biology team within the Oncology Thematic Research Center in Cambridge, MA.

Responsibilities

• Applies extensive scientific knowledge and technical skills to conduct molecular and cellular biology related studies to better understand target and disease biology
• Uses current and new technologies for function-based characterization for small molecule hit validation, mechanism of action understanding, and compound triaging/ranking
• Designs and executes experiments independently, troubleshoots as needed, interprets and discusses data, with accurate, timely and detailed record-keeping
• Performs laboratory work related but not limited to 2D/3D/spheroid cell culture and related cellular assays, Western blot and/or JESS, qRT-PCR, flow cytometry, proliferation and/or cytotoxicity assays (CyQuant, CTG, apoptosis, IncuCyte, etc.), high-content imaging (e.g. Phenix), ELISA, MSD and/or HTRF assays, colony formation assay, processing of DNA/RNA/protein, IP/Co-IP
• Generates and characterizes relevant cell models, such as using genetic tools (CRISPR, shRNA, siRNA, lentiviral mediated target overexpression, etc.) and establishing drug-resistant cell lines
• Collaborates with rest of biology team members as well as other functional area leads e.g. pharmacology, DMPK, informatics and predictive science, etc.
• Communicates via email, meetings, and reports
• Makes presentations in small, multidisciplinary meetings, logically presenting data and interpretation to convey clear, key messages

Qualifications

• BS with 5 years of experience post-graduation or MS
• Expertise in mammalian cell culture, plate-based cellular assays, immunoblotting, lentiviral infection, cellular transfection, and basic molecular biology techniques
• Experience with medium and high-throughput screening is highly preferred
• Experience with targeted protein degradation assay is a plus
• Familiarity with automation instruments and multi-mode plate readers
• Outstanding problem-solving and organization skills
• Effective team player and strong individual contributor
• Self-driven, flexible, pragmatic, and able to handle multiple tasks while delivering high quality data
• Effective communication skills, both verbal and written