Scientist Antibody Discovery

Join an excellent team of researchers dedicated to coming closer to the mission of St. Jude Children’s Research Hospital: that no child will die at the dawn of life. The Antibody Discovery Collaborative Centre (ADCC) within the Department of Host Microbe Interactions seeks a skilled and highly motivated candidate experienced in antibody discovery. Candidates with experience in antibody discovery, protein engineering, or expression and validation of biologics are encouraged to apply.

Responsibilities

• Antibody discovery:
  • Focus on display methods (phage, yeast, mammalian) and experience in building high-diversity libraries from synthetic and animal repertoires.
  • Previous experience in integrating ML-based approaches with display methods would be preferred.
  • Design discovery workflows and deliver panels of lead candidates for programs.
  • Apply in-vitro display methods for sequence discovery and handle multiple screening arms for antigens.
  • Apply NGS methods to mine antibody pools for sequence discovery.
  • Previous experience in library generation would be vital.
• Protein Sciences:
  • Develop and optimize high-throughput mammalian cell-based production platforms for recombinant antibodies, antigens, and other biologics.
  • Work closely with mAb discovery platforms to support molecular biology workflows; design and execute construct generation for antibody expression.
  • Perform DNA preparations to generate high-quality plasmid material for mammalian cell transfection.
  • Establish scalable transient and stable expression workflows in CHO, HEK293, and other mammalian expression systems.
  • Purification using AKTA chromatography systems.
  • High-Performance Liquid Chromatography Size Exclusion Chromatography (HPLC-SEC) analysis of purified proteins.
  • Molecular assessment of early-stage development candidates (polyreactivity ELISA, CE-SDS, SEC-MALS, DSF).
  • Maintain accurate and timely ELN data.

Preferred Skills

• Experience with merging cryo-EM techniques with epitope analysis of antibody repertoires.
• Experience with high-throughput automated protein purification systems (e.g., Kingfisher).
• Experience with antibody-related bioinformatic/software packages.
• Expertise in high-throughput molecular biology approaches for construct and library generation.
• Experience with mAb characterization techniques (FACS, BLI, epitope binning).

Requirements

• Bachelor's degree in a relevant scientific area is required.
• Minimum experience:
  • Eight (8) years of relevant work experience is required.
  • Six (6) years of relevant work experience is required with a Master's degree.
  • Three (3) years of relevant work experience is required with a PhD.

Pay

A reasonable estimate of the current salary range is $86,320 - $154,960 per year.

Benefits

Explore our exceptional benefits.