Research Technician III

About the role

The David Lab at Duke University is recruiting a Research Technician III to support the development and use of new DNA sequencing tools to study diet and gut microbiome. The position is based on-site and involves running molecular workflows, maintaining lab records, and collaborating with researchers and communities.

Responsibilities

• Receive and process wastewater samples, mail-in citizen-science kits, and samples from collaborator laboratories; verify metadata, barcodes, temperature, and control logs.
• Accomplish DNA extractions from concentrates, swabs, and solids, quantify nucleic acids, and prepare amplicon libraries (e.g., trnL, 12SV5) and other targeted assays with accuracy and consistency.
• Set up and run PCR and related workflows using standard instruments (thermocyclers, fluorometers, plate readers), controls, and standards.
• Prepare sequencing libraries and support sequencing workflows, including coordination with internal or external sequencing resources as needed.
• Troubleshoot assays, identify sources of technical variation, make minor modifications to established protocols as needed, and document deviations and corrective actions.
• Summarize quality-control metrics and experimental results for project tracking, reporting, and downstream analysis.
• Prepare buffers, reagents, and other lab materials; track lot numbers and manage inventory; place orders and receive supplies.
• Calibrate and maintain common equipment; coordinate preventative maintenance and service calls.
• Operate and maintain laboratory robotics and automated liquid handling systems.
• Uphold biosafety, chemical hygiene, and waste-disposal procedures.
• Maintain and improve standard operating procedures and protocols.
• Conduct routine, detail-intensive experiments with enthusiasm, constantly seeking ways to improve speed and efficiency without compromising quality.
• Assist in collecting, processing, and managing data from various genomic experiments.
• Coordinate with field sampling teams on field sampling needs, sample readiness, and turnaround.
• Train and help oversee student assistants and other junior personnel on core laboratory workflows, safety procedures, documentation standards, quality expectations, and timelines.
• Work collaboratively within the lab team and contribute to a positive and productive research environment.

Requirements

• Minimum requirements: Bachelor's degree in botany, biology, zoology, psychology or other science-related fields. Two years of research experience or a related master's degree in lieu of two years of experience.
• Preferred qualifications: Experience with DNA extraction, PCR, sequencing-library preparation, and/or laboratory automation.

Qualifications

• Technical proficiency: Experience in genomics or related fields, with a background in DNA extraction, PCR, and/or sequencing.
• Growth mindset: Strong willingness to learn, adapt, and develop new skills. Curiosity and proactive approach to problem-solving.
• Dedication and efficiency: Passion for experimental work, focus on precision and efficiency, self-motivation, and dedication to continuous improvement.
• Communication skills: Ability to communicate effectively within a team and contribute to a collaborative research environment.

Skills

• Experience with DNA extraction, PCR, sequencing-library preparation, and/or laboratory automation.
• Strong willingness to learn, adapt, and develop new skills.
• Passion for experimental work, focus on precision and efficiency.
• Effective communication within a team and contribution to a collaborative research environment.

Benefits

The David Lab at Duke University offers a stimulating environment where your contributions will have a meaningful impact on scientific research and public health. We welcome applicants who are passionate about advancing genomic technologies and are ready to embark on a challenging and rewarding research career.

Pay

The anticipated pay range for this position is USD $22.61 to USD $34.27 annually. The university considers factors such as (but not limited to) scope and responsibilities of the position; candidate's work experience, education/training, and key skills; internal peer equity; as well as market and organizational considerations when extending an offer.