Research Lab Technician Lead

Responsibilities

• Perform molecular biology assays, including DNA extraction, RNA extraction, genotyping, PCR, gel electrophoresis, biochemical assays, recombinant DNA cloning, and qRT-PCR.
• Generate cardiomyocyte tissues and perform microscopy on these cardiac tissues - this includes culture of iPSC-derived cardiomyocytes, delivery of viral vectors and treatments, and fabrication of engineered substrates for tissues.
• Perform live cell microscopy of human cardiac tissues, immunostaining and imaging of fixed cardiac tissues, subsequent image analysis and quantification using standardized pipelines developed in the lab.
• Routine laboratory maintenance including ordering and restocking, biohazard waste autoclaving and disposal, and dishwashing.
• Back-up other positions in the lab, including routine iPSC culture and mouse colony maintenance.
• Regularly communicate and coordinate with other team members to perform experiments in mouse models. Participate in weekly lab meetings.

Required Qualifications

• A minimum of a bachelor's degree in a biological or related field.
• At least 1 year of prior laboratory experience is required.
• Prior experience with basic molecular biology techniques, including PCR and genotyping techniques, is required.
• Ability to perform precise fine motor manipulations of engineered substrates is required (although no specific experience with these fabrications is required as training in the techniques will be provided).
• Excellent organizational skills, perseverance, the ability to keep detailed notes, meticulous attention to details, excellent verbal and written English language skills, and an eagerness to work as an integrated team player which may include teaching rotating students and fellows.
• A minimum 2-year commitment is also requested.

Desired Qualifications

• Cell culture and/or microscopy experience are desired but not mandatory (training in all of the above techniques will be given).