Research Biologics Technologist 1

Responsibilities

• Follow pre-defined protocols, policies, procedures, regulations, Good Clinical & Good Laboratory Practices.
• Conduct standard and repetitive experiments and complete basic trouble-shooting techniques.
• Set up, operate, and maintain standard lab equipment.
• Record test results and maintain databases; perform basic statistical analyses.
• Complete standard and routine clerical duties such as copying, filing, data entry, and word processing.
• Maintain inventory of reagents, mice, and lab supplies.
• Perform the following activities with an entry-level skill set:
  • Molecular Biology and Biochemistry:
    • DNA, RNA, Protein isolation, purification, and expression
    • Southern, Northern, Western blotting
    • PCR, RT-PCR
    • Plasmid preparation
    • Cloning, mapping, and sequencing of DNA; single nucleotide polymorphisms detection
    • Recombinant virus production and purification
    • ELISA assays
    • HPLC
    • Mitochondria functional analysis (Oroboros)
  • Cellular Biology:
    • Cell isolation, cell culture, and co-culture
    • Organoids culture
    • Functional studies
    • Transfection and transduction
    • Flow cytometry
  • Histology and Immunohistochemistry:
    • Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
    • Immunohistochemistry
    • Microscopy
    • Tissue quantification
  • Animal:
    • Husbandry
    • Breeding and screening transgenic mice
    • Dissections
    • Blood collection
    • Surgical procedures
    • Database management of colony
    • Physical and cognitive functional assays
  • Organ and Tissue Physiology:
    • Making physiological solutions
    • Tissue dissection
    • In vitro physiological assays
  • Electronic Equipment: Calibration and use
• Complete other duties as assigned.

Qualifications

• High School diploma or equivalent; some basic science courses required.
• Excellent oral and written communication skills in English.
• Strong interpersonal and organizational skills needed to participate as a creative member of a research team.
• Ability to exchange ideas and work closely with clinicians, physical and occupational therapists, patients, and professional organizations.
• Ability to timely plan and coordinate project completions.

Working Conditions

• Laboratory-based position with substantial time spent conducting experiments at the bench.
• Office setting for data analysis, writing, and administrative tasks.
• Need for protective gear, such as lab coats, gloves, and safety glasses, depending on the experimental procedures and hazards involved.

Reports directly to the assigned Principal Investigator.