Research Associate III - Spatial Technology Platform

Description

The Broad Institute of MIT & Harvard, a world leader in genetic research, is seeking a highly motivated individual to join the imaging group within the Spatial Technology Platform (STP). STP utilizes cutting-edge spatial profiling methods to generate highly multiplexed and multi-modal data sets at single-cell resolution as a high-throughput scaled service at the Broad Institute. The platform also develops and pilots new technologies and capabilities that have potential for scaled service implementation, including cross-modality assays and technologies at precommercial stages.

Requirements

• Develop and evaluate methods to assess quality of input material for spatial transcriptomics assays - RNA extraction and DV200 profiling, H&E imaging, and RNAscope profiling.
• Pilot methods to adapt spatial transcriptomics workflows for high-throughput profiling of cultured cells.
• Implement imaging-based spatial profiling methods such as 10X Xenium in combination with other assays such as RNAscope and multiplexed immunofluorescence.
• Support the platform’s self-service imaging instrumentation through user training of widefield, confocal, and light sheet microscopy.
• Attention to detail when troubleshooting and resolving issues in assays; ability to propose next steps or directions even when the path to an experimental goal is not clear.
• Support of histology needs.
• Flexibility in a rapidly changing environment.
• Document, compile, and analyze experimental data.
• Attend and present at team meetings to share results and ensure that projects support team goals.