Research Associate III

Responsibilities

• Execute and troubleshoot production and QC of neuronal cultures for Quiver’s multiple customer-based and internal R&D projects.
• Maintain and characterize multiple human iPS cell lines.
• Use transcriptional programming and directed differentiation strategies to produce various types of high-quality human iPS cell-derived neurons, including excitatory, inhibitory, sensory, and motor neurons.
• Plate and maintain cultures of human iPS cell-derived neurons in 96-well and 384-well assay formats.
• Carry out RNA and DNA preparations from human cell lines and cultured neurons.
• Perform candidate gene expression analysis using quantitative PCR assays.
• Assess protein expression using immunocytochemistry (ICC) and immunoblotting assays.
• Assist with cell culture lab operations, including inventory, purchasing of lab reagents and supplies, and aliquoting of recombinant protein and lentiviral particle stocks.
• Communicate work progress via technical reports, PowerPoint presentations, and seminars at internal meetings.

Requirements

• Master’s degree in biology, cell biology, biotechnology, or a related field.
• At least two years of experience with mammalian cell culture (in particular iPS cell-derived neurons) and with DNA/RNA/Protein preparations and readout assays (PCR, qPCR, immunoblotting, and ICC).
• Ability to work independently and in cross-disciplinary teams.
• Excellent oral, written, and interpersonal communication skills.

Preferred Qualifications

• Experience with methods for human iPS cell differentiation into neurons.
• Experience with 96/384-well cultures and high-throughput screening.
• Experience with neuronal preparations from rodent CNS tissues.