Research Associate - Anesthesia, Obal's Lab

About the role

The Department of Anesthesia is seeking a full-time Research Associate to participate in a cardiovascular research project. The research primarily utilizes patient-specific induced pluripotent stem cell-derived cardiomyocytes to model cardiac disorders.

Responsibilities

• Induced pluripotent stem cell reprogramming and culture.
• Cardiomyocyte and endothelial differentiation experiments using human iPSCs.
• Perform cardiomyocyte and endothelial cell phenotype and molecular evaluation protocols.
• Subcutaneous injection of drugs in pregnant mice.
• Plan and perform existing experiments in support of ongoing research projects based on extensive knowledge of scientific theory.
• Interpret and perform analyses of results and their possible implications.
• Discuss issues and considerations for using different types of research approaches.
• Analyze and interpret literature on an ongoing basis to remain current with new procedures and related research, interpret new findings and analyze their validity.
• Demonstrate in depth knowledge of and offer suggestions regarding modifications to procedures and protocols.
• Advise on advanced research analysis methods.
• Maintain detailed records of experiments and outcomes.
• Design, write, and publish findings as needed.
• Participate in the preparation of written documents, including procedures, presentations, and proposals.
• Edit and revise as needed.
• Care for general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and coordinate lab support when required.
• Other duties as assigned.

Qualifications

• Bachelor's degree in related scientific field, or an equivalent combination of education and experience.
• One year of related experience is required.
• Extensive understanding of scientific principles.
• Demonstrated performance to use knowledge and skills when needed.
• Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
• Demonstrated ability to maintain detailed records of experiments and outcomes.
• General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
• Ability to work under deadlines with general guidance.
• Excellent organizational skills and demonstrated ability to accurately complete detailed work.

Desired Qualifications

• A Bachelor of Science in molecular biology or related discipline or equivalent experience.
• Two year’s experience.
• Technical writing experience in SOP’s, protocols, and reports.
• Proficiency with human cell culture techniques, experience with iPSC is highly valued.
• Experience with laboratory animal experiments, in particular, s.c. injection of drugs.
• Experience with tissue homogenization and single cell preparation from various tissues, primarily cardiomyocytes.
• Proficiency with basic molecular biology techniques, such as gel electrophoresis, PCR, and recombinant DNA techniques.
• Routinely perform cardiomyocyte and endothelial cell differentiation experiments using human iPSCs.
• Possess initiative, ability to multi-task and a desire to acquire new technical skills.
• Ability to work both independently and as part of a team.
• Ability to communicate effectively with team members and collaborators.
• Experimental design, execution of plan and presentation of data to team.
• Utilization of good documentation practices.