Research Assistant - Anesthesia, Obal's Lab

About the role

The Department of Anesthesia is seeking a full-time Research Assistant to participate in a cardiovascular research project. The research primarily utilizes patient-specific induced pluripotent stem cell-derived cardiomyocytes to model cardiac disorders.

Responsibilities

• Induced pluripotent stem cell reprogramming and culture
• Cardiomyocyte and endothelial differentiation experiments using human iPSCs
• Perform cardiomyocyte and endothelial cell phenotype and molecular evaluation protocols
• Subcutaneous injection of drugs in pregnant mice
• Read and understand existing experiments in support of ongoing research projects based on knowledge of scientific theory
• Interpret and perform analyses of results
• Review literature on an ongoing basis to remain current with new procedures and related research
• Offer suggestions regarding modifications to procedures and protocols
• Maintain detailed records of experiments and outcomes
• Contribute to publication of findings as needed
• Participate in the preparation of written documents, including procedures, presentations, and proposals
• Assist with general lab maintenance as needed; assist with maintaining lab stock, assist with management of chemical inventory and safety records, and provide general lab support as needed
• Other duties as assigned

Qualifications

• Education Requirements: Bachelor's degree in related scientific field, or an equivalent combination of education and experience
• Experience Requirements: General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed. Demonstrated ability to apply theoretical knowledge of science principals to problem solve work. Ability to maintain detailed records of experiments and outcomes. General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications. Ability to work under deadlines with general guidance. Excellent organizational skills and demonstrated ability to accurately complete detailed work.
• Desired Qualifications: A Bachelor of Science in molecular biology or related discipline or equivalent experience Two year’s experience Technical writing experience in SOP’s, protocols, and reports Proficiency with human cell culture techniques, experience with iPSC is highly valued Experience with laboratory animal experiments, in particular, s.c. injection of drugs Experience with tissue homogenization and single cell preparation from various tissues, primarily cardiomyocytes Proficiency with basic molecular biology techniques, such as gel electrophoresis, PCR, and recombinant DNA techniques Routinely perform cardiomyocyte and endothelial cell differentiation experiments using human iPSCs Possess initiative, ability to multi-task and a desire to acquire new technical skills Ability to work both independently and as part of a team Ability to communicate effectively with team members and collaborators Experimental design, execution of plan and presentation of data to team Utilization of good documentation practices