Research Assistant III

Responsibilities

• Execute neutralizing antibody (nAb) and total antibody assays on clinical (human) and non-clinical (animal/cell) samples.
• Maintain cell lines required for live-virus or pseudovirus neutralization assays.
• Handle high-throughput manual pipetting.
• Formulate buffers, cell culture media, and assay reagents according to Standard Operating Procedures (SOPs).
• Maintain strict adherence to CLIA, GCLP, and OSHA safety standards.
• Execute positive and negative controls for every assay batch to ensure data validity.
• Clean, calibrate, and maintain microplate readers, incubators, and biosafety cabinets.
• Complete precise batch records, sample logs, and equipment maintenance sheets.
• Accession, aliquot, and track clinical serum or plasma samples using a LIMS system.
• Track expiration dates and stock levels of critical assay components like reference standards.
• Collect raw data (e.g., fluorescence, or absorbance) from plate readers.
• Calculate neutralization titers (like IC50/EC50) and total antibody concentrations for review by the Lab Director.
• Neutralize and dispose of biohazardous liquid and solid waste according to regulations.

Requirements

• 5+ years of experience.
• Minimum of a Bachelor's degree in related field.
• Master's in related field preferred.
• Experience with Microsoft office suite.
• LIMS experience is required.
• Experience with LabVantage is preferred.
• Experience in a CLIA or regulated laboratory required.
• Experience working with Immunological assays.
• Experience with ELISA is strongly preferred.