Research Assistant II Neurology KL

About the role

The candidate will work independently under the supervision of Vikram Khurana, MD, PhD, and a senior researcher in the Khurana lab (instructor/assistant professor).

Responsibilities

• Maintenance, passaging, cataloguing, and culturing of iPSC cells.
• Differentiation iPSCs to neurons and glia and aging of these cultures.
• Maintenance of Attune flow cytometry and ordering the related reagents.
• Measuring and scheduling of longitudinal imaging with Incucyte and Nikon Biostation microscope.
• Maintenance of HEK293 cells, generation of stable cell lines.
• Helping with molecular cloning; Gibson assembly, gateway cloning, mini/maxi preps.
• Analysis of data from western blot/ microscopy/ flow cytometry.
• Excellent lab note-keeping habits, mostly digital (LabArchieves) and generating Standard Operating Procedures (SOPs) for the lab.
• Excellent organizational skills, labeling of experiments, file management.
• Reporting daily to the immediate supervisor and weekly to the Principal Investigator.
• Interacting with junior and senior members of the lab to troubleshoot experiments.
• Sharing lab duties with other technicians.
• Responsible for data collection, management, manipulation, analysis, and reporting.
• Cohort management.
• Outreach communication with subjects, conducting mailings, follow-up on mailings, and obtaining necessary documents.
• Provides assistance on work related to epidemiological studies and other population-based research.
• Performs other duties as assigned.
• Complies with all policies and standards.

Qualifications

• Bachelor's Degree in Science required.
• One year of post grad research experience.
• Can this role accept experience in lieu of a degree? Yes.
• New grad with some relevant coursework 0-1 year preferred.
• Good cell culture experience with human cells (HEK293 or any other immortalized line).
• iPSC stem cell culture experience is a plus but not required.
• Excellent cell culture practice/sterile techniques are necessary.
• Moderate to high level of computer literacy and a basic understanding of statistical concepts are needed.
• Basic understanding of molecular techniques, microscopy, flow cytometry is desired.
• Understanding of basic molecular concepts: molecular cloning, western blot, RNA isolation, qPCR, western blot is desired.

Knowledge, Skills And Abilities

• Excellent oral and written communication skills.
• Strong interpersonal skills are required for working in a highly diverse lab.
• Excellent organizational skills and ability to prioritize a variety of tasks.
• Excellent data management skills and attention to detail.
• Good presentation skills are a plus.
• Computer literacy, including database tools.
• Ability to follow directions and exhibit professionalism.