Research Assistant II - GI Medical Oncology

Responsibilities

• Molecular Techniques
• Perform DNA, RNA, and protein extraction procedures.
• Measure DNA, RNA, and protein concentrations using established laboratory methods.
• Conduct DNA, RNA, and protein gel electrophoresis experiments.
• Execute real-time quantitative RT-PCR assays.
• Generate knockout cell lines for research studies.
• Utilize antibodies in immunostaining applications.
• Perform indirect immunofluorescence techniques for flow cytometry.
• Perform immunohistochemistry procedures.
• Support bioengineering studies involving microfluidics chips, neuromodulation, and intravital imaging.
• Tissue Processing, Histology & Specimen Management
• Receive human tissue specimens for research use.
• Log and track tissue specimens using established documentation procedures.
• Process human tissue specimens for laboratory research activities.
• Conduct enzymatic and mechanical tissue dissociation procedures.
• Isolate cells from human tissue specimens.
• Prepare biological specimens harvested from mouse models.
• Dissect mice for research-related tissue collection.
• Fix and embed tissues for histological analysis.
• Perform immunostaining procedures on tissue specimens.
• Cell Culture & Experimental Support
• Maintain tissue cultures for ongoing research studies.
• Maintain 3D patient-derived organoid cultures.
• Maintain microfluidics-based microtissues.
• Maintain various immune cell cultures.
• Perform cryopreservation and recovery of cryopreserved human tissue-derived cells.
• Maintain established cell lines using standard tissue culture techniques.
• Plate cells and monitor cell growth.
• Determine cell counts and evaluate cell viability.
• Perform transient and stable transfections, proliferation assays, and functional assays.
• Data Management, Documentation & Communication
• Maintain accurate and detailed records of experimental data.
• Maintain accurate and detailed records of laboratory protocols.
• Analyze research data using computer applications.
• Organize and plot research data for interpretation and reporting.
• Enter experimental and specimen data into research databases.
• Maintain accurate specimen tracking documentation.
• Communicate experimental results to supervisors and laboratory personnel.
• Report potential experimental issues and research challenges promptly.
• Laboratory Operations, Safety & General Support
• Maintain a clean, safe, and orderly laboratory environment.
• Follow verbal and written instructions accurately.
• Comply with all institutional policies and OSHA regulations regarding biohazard and radiation safety.
• Handle hazardous chemicals according to safety protocols.
• Operate and maintain various laboratory equipment and apparatus.
• Prepare media, solutions, and reagents accurately.
• Apply basic scientific terminology and cancer research concepts in daily laboratory activities.
• Perform literature and online database searches to retrieve scientific information.
• Learn new techniques, collaborate with staff and research partners, and assist with additional laboratory activities as needed.

Qualifications

• Experience or exposure to molecular biology techniques, tissue processing, cell culture, immunostaining procedures, and research documentation.
• Strong scientific aptitude, organizational skills, and a willingness to learn new laboratory techniques while collaborating effectively with research staff and investigators.