Research Assistant I - Pharmacology and Molecular Therapeutics

Responsibilities

• Assists in designing, developing, executing, and implementing biochemical and cell biological experiments related to a new research projects assessing the role of WT RAS activation in RAS-mutated cancers.
• Investigates the feasibility of applying a wide variety of scientific principles and theories to potential inventions and products.
• Performs specialized laboratory research utilizing experimental protocols which will involve specialized procedures including: cell line generation using both viral vectors and CRISPR technology, cell culture, nucleic acid extraction/purification/transfection, growth factor stimulation, BRET, immunoprecipitation and Western blotting, molecular cloning and site-directed mutagenesis, immunofluorescence and confocal microscopy, animal genetics and husbandry, and other techniques as needed/required by the laboratory.
• Colls collects and handles samples (mice and rats) and keeps detailed records of experiments.
• Processes and interprets experimental data, and prepares accurate and timely research reports.
• Aids in the preparation of data for presentations at scientific meetings and for publication in journals.
• Aids with the training of laboratory staff as needed.
• Maintains cleanliness of laboratory areas and performs routine maintenance tasks on laboratory equipment.
• Performs flow cytometry to assess CSC markers in DTPs.
• Performs and analyzes ssRNA sequencing.
• Acquires RTK-mutated NSCLC cell lines from ATCC.
• Performs other duties as assigned.

Qualifications

• Education and Experience: Bachelor's Degree required, preferably in Biology, Biochemistry, Molecular Biology, Cancer Biology, Pharmacology, or a related discipline. Minimum of 0-2 years experience required.
• Required Knowledge, Skills And Abilities: Knowledge of standard laboratory techniques. Experience with use of personal computers for manuscript preparation and data analysis is essential. Additional training will be provided for advanced animal experimental techniques. Subject knowledge in biology, biochemistry, and genetics. Laboratory experience with competence in routine areas of modern cellular and molecular biology. Ability to follow detailed instructions. Good communication and analytical skills. Ability to obtain and maintain a T1 Public Trust background check.
• Physical Capabilities: Lifting: Requires lifting materials up to 25 lbs. Ability to stand or sit at a computer for prolonged periods.