Process Development Associate I - PRISM Cell Pooling

About the role

The Broad Institute is seeking an outstanding candidate for the position of Process Development Associate I within the PRISM team, a component of the Cancer Program. Dedicated to transforming medicine, the Broad Institute works to significantly accelerate the understanding and treatment of disease. As part of this important mission, the Cancer Program is advancing the understanding of oncology therapeutics through the systematic profiling of oncology therapeutics using the PRISM technology of large-scale screening of more than 900 cell lines.

Responsibilities

• Manage cell culture for up to 30 cell lines simultaneously to generate PRISM pools
• Maintain our cell bank of 1,000 cell lines, including barcoding new cell lines, creating new freezer stocks of cell lines, and regularly updating inventories/databases
• Execute large-scale cell plating for production screens
• Conduct molecular biology protocols and related quality control assays on cell lines, including PCR, DNA/RNA extraction, gel electrophoresis for mycoplasma testing, sequence submission, and STR testing sample shipments
• Execute CTG cell viability assays as needed
• Maintain comprehensive lab records, experimental write-ups, and processing notes using an electronic lab notebook (eLabNext)
• Prepare and maintain stocks of reagents, solutions, and lab supplies and manage inventories
• Perform routine maintenance on lab equipment to ensure optimal equipment functionality (e.g., plate washers, combi liquid dispensers, etc.)
• Maintain and update SOPs to accurately reflect any changes in production protocols
• Assess the quality of PRISM cell pools utilizing an internal data visualization tool
• Participate in team meetings to share results, plan projects, and ensure alignment with current team goals

Minimum Qualifications

• BS/BA in Biology, Biomedical Engineering or related fields
• 1+ year experience in a biological research environment, additional experience with mammalian cell culture and aseptic technique is preferred
• Experience with basic molecular biology techniques, including PCR, DNA extractions, etc.
• Excellent written and oral communication skills and ability to effectively work as part of a team
• Strong interpersonal, organizational, and analytical skills, including the ability to handle a variety of tasks in a fast-paced environment
• Strong attention to detail, time management skills, and the ability to maintain accurate records and documentation

Pay

The expected base pay range for this position is $50,000.00/yr – $70,000.00/yr for a 40-hour work week. Actual compensation will vary based on factors including, but not limited to, relevant skills, experience, education, qualifications, and other factors permissible by law.