Postdoctoral Fellow/ Scientist I, Single-Cell Method Development

About the role

The Laboratory of Immunogenomics at CZ Biohub NY is seeking a highly motivated Postdoctoral Fellow/ Scientist I to lead the development of a high-throughput adaptation of a newly established single-cell nascent RNA sequencing method (scGRO-seq) and apply it to patient biopsies and organoid models.

Responsibilities

• Assay development: Design, optimize, and scale high-throughput single-cell/nuclei extraction, barcoding, and library preparation workflows for scGRO-seq.
• Perturbation integration: Combine scGRO-seq with CRISPR-based enhancer perturbation, drug treatments, and multi-omics profiling.
• Molecular biology techniques: Execute advanced methods including assay design, cell manipulation, RNA/DNA isolation, PCR/qPCR, NGS library construction, high-dimensional flow cytometry, and sequencing.
• Translational applications: Adapt and validate protocols in patient biopsies and 3D organoid models, with emphasis on reproducibility and sensitivity.
• Immune cell engineering: Isolate, culture, and reprogram hematopoietic stem/progenitor cells (HSPCs) and primary immune subsets.
• Data integration: Partner with computational biologists to process and interpret large-scale single-cell datasets (RNA, chromatin, proteomics).
• Collaboration & dissemination: Collaborate across disciplines to investigate regulatory genomics in immune dysfunction, tumor-infiltrating immune cells, and autoimmune disease variants. Contribute to publications, preprints, conference presentations, and grant proposals.

Requirements

• PhD in Biomedical Engineering, Molecular Biology, Systems Biology, Genetics, or related field.
• 4+ years of experience working in genomics assay development or method optimization.
• 2+ years of experience working on single-cell transcriptomics, droplet chemistry, and microfluidics.
• Proven expertise and demonstrated success in bulk or single-cell method development.
• Experience designing and executing CRISPR screens and perturbation assays (genetic or chemical/drug).
• Strong problem-solving skills, independence, and the ability to troubleshoot complex workflows.
• Excellent communication skills and proven ability to work in collaborative, multidisciplinary teams.

Qualifications

• Experience and broad domain knowledge in microfluidic-based single-cell methods.
• Experience with hydrogel synthesis, single-cell barcoded bead generation, and aqueous droplets in oil emulsions.
• Experience with functional genomics in immune cells.
• Experience integrating multi-modal single-cell datasets (RNA, ATAC, proteomics).
• Working knowledge of Linux/HPC environments and single-cell analysis tools (e.g., Seurat, Scanpy).

Skills

• Strong background in molecular biology and biochemistry.
• Experience with single-cell sequencing technologies and bioinformatics tools.
• Ability to work independently and as part of a team.
• Excellent organizational and problem-solving skills.

Benefits

• Provides a generous employer match on employee 401(k) contributions to support planning for the future.
• Paid time off to volunteer at an organization of your choice.
• Funding for select family-forming benefits.
• Relocation support for employees who need assistance moving.

Pay

The New York, NY base pay for a new hire in this role is Postdoctoral Fellow = $93,000.00, Scientist I = $120,000 - $150,000. This position may be eligible to participate in Biohub's discretionary annual performance bonus program. Bonus eligibility and targets are determined in accordance with Biohub's total rewards philosophy and may vary by role.